PMID- 25051246
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20191210
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 139
IP  - 18
DP  - 2014 Sep 21
TI  - Chemically modified flexible strips as electrochemical biosensors.
PG  - 4661-72
LID - 10.1039/c4an00646a [doi]
AB  - A flexible and disposable strip sensor for non-enzymatic glucose detection is 
      demonstrated in this work. The strips are prepared by using chemical modification 
      processes followed by a simple electroless deposition of copper. Essentially, 
      polyester overhead projector (OHP) transparent films are modified with a 
      monolayer of 3-aminopropyltrimethoxysilane (APTMS) and polyaniline (PANI) 
      conducting polymer. Later, nanostructured copper is deposited onto this modified 
      film. Scanning electron microscope (SEM) and X-ray diffraction (XRD) studies are 
      used for the structural, morphological and crystallinity characterization of the 
      modified films. Electrochemical techniques, namely cyclic voltammetry (CV) and 
      chronoamperometry (CA), are employed for the non-enzymatic detection of glucose. 
      These studies clearly reveal the formation of homogeneous, close-packed spherical 
      Cu particles converged into uniform film that exhibits a good catalytic activity 
      towards the oxidation of glucose. The Cu/PANI/APTMS/OHP sensor displays a 
      remarkable enhancement in the oxidation current density, a very high sensitivity 
      value of 2.8456 mA cm(-2) per mM, and a linear concentration range from 100 muM to 
      6.5 mM associated with glucose detection. Detection limit is estimated to be 5 muM 
      and the response time of the sensor is determined to be less than 5 s. For 
      comparison, similar studies are performed without PANI, namely Cu/APTMS/OHP films 
      for glucose detection. In this case, a sensitivity value of 2.4457 mA cm(-2) per 
      mM and a linear concentration range of 100 muM-3 mM are estimated. The higher 
      performance characteristics observed in the case of Cu/PANI/APTMS/OHP are 
      attributed to the synergistic effects of the conducting polymer acting as an 
      electron facilitator and the nanostructured Cu films. These disposable, flexible 
      and low-cost strip sensors have also been applied to the detection of glucose in 
      clinical blood serum samples and the results obtained agree very well with the 
      actual glucose level.
FAU - Thota, Raju
AU  - Thota R
AD  - Electrodics and Electrocatalysis (EEC) Division, Council of Scientific and 
      Industrial Research-Central Electrochemical Research Institute (CSIR-CECRI), 
      Karaikudi, 630006, Tamilnadu, India. vganesh@cecri.res.in ganelectro@gmail.com.
FAU - Ganesh, V
AU  - Ganesh V
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aniline Compounds)
RN  - 0 (Blood Glucose)
RN  - 0 (Propylamines)
RN  - 0 (Reagent Strips)
RN  - 0 (Silanes)
RN  - 0 (polyaniline)
RN  - 13822-56-5 (3-aminopropyltrimethoxysilane)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Aniline Compounds/*chemistry
MH  - Biosensing Techniques/instrumentation
MH  - Blood Glucose/*analysis
MH  - Copper/*chemistry
MH  - Electrochemical Techniques/*instrumentation
MH  - Humans
MH  - Limit of Detection
MH  - Propylamines/*chemistry
MH  - Reagent Strips/*analysis
MH  - Silanes/*chemistry
EDAT- 2014/07/23 06:00
MHDA- 2015/08/25 06:00
CRDT- 2014/07/23 06:00
PHST- 2014/07/23 06:00 [entrez]
PHST- 2014/07/23 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - 10.1039/c4an00646a [doi]
PST - ppublish
SO  - Analyst. 2014 Sep 21;139(18):4661-72. doi: 10.1039/c4an00646a.