PMID- 25051430
OWN - NLM
STAT- MEDLINE
DCOM- 20150818
LR  - 20141009
IS  - 1525-6073 (Electronic)
IS  - 0742-0528 (Linking)
VI  - 31
IP  - 9
DP  - 2014 Nov
TI  - Repeated psychosocial stress at night, but not day, affects the central molecular 
      clock.
PG  - 996-1007
LID - 10.3109/07420528.2014.940085 [doi]
AB  - We have recently demonstrated that the outcome of repeated social defeat (SD) on 
      behavior, physiology and immunology is more negative when applied during the 
      dark/active phase as compared with the light/inactive phase of male C57BL/6 mice. 
      Here, we investigated the effects of the same stress paradigm, which combines a 
      psychosocial and novelty stressor, on the circadian clock in transgenic 
      PERIOD2::LUCIFERASE (PER2::LUC) and wildtype (WT) mice by subjecting them to 
      repeated SD, either in the early light phase (social defeat light = SDL) or in 
      the early dark phase (social defeat dark = SDD) across 19 days. The PER2::LUC 
      rhythms and clock gene mRNA expression were analyzed in the suprachiasmatic 
      nucleus (SCN) and the adrenal gland, and PER2 protein expression in the SCN was 
      assessed. SDD mice showed increased PER2::LUC rhythm amplitude in the SCN, 
      reduced Per2 and Cryptochrome1 mRNA expression in the adrenal gland, and 
      increased PER2 protein expression in the posterior part of the SCN compared with 
      single-housed control (SHC) and SDL mice. In contrast, PER2::LUC rhythms in the 
      SCN of SDL mice were not affected. However, SDL mice exhibited a 2-hour phase 
      advance of the PER2::LUC rhythm in the adrenal gland compared to SHC mice. 
      Furthermore, plasma levels of brain-derived neurotrophic factor (BDNF) and BDNF 
      mRNA in the SCN were elevated in SDL mice. Taken together, these results show 
      that the SCN molecular rhythmicity is affected by repeated SDD, but not SDL, 
      while the adrenal peripheral clock is influenced mainly by SDL. The observed 
      increase in BDNF in the SDL group may act to protect against the negative 
      consequences of repeated psychosocial stress.
FAU - Bartlang, Manuela S
AU  - Bartlang MS
AD  - Department of Neurobiology and Genetics, Biocenter, University of Wurzburg , 
      Wurzburg , Germany .
FAU - Savelyev, Sergey A
AU  - Savelyev SA
FAU - Johansson, Anne-Sofie
AU  - Johansson AS
FAU - Reber, Stefan O
AU  - Reber SO
FAU - Helfrich-Forster, Charlotte
AU  - Helfrich-Forster C
FAU - Lundkvist, Gabriella B S
AU  - Lundkvist GB
LA  - eng
GR  - IR21DC013172/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140722
PL  - England
TA  - Chronobiol Int
JT  - Chronobiology international
JID - 8501362
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Period Circadian Proteins)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Circadian Rhythm/*physiology
MH  - Gene Expression Regulation
MH  - *Light
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Period Circadian Proteins/metabolism
MH  - Periodicity
MH  - Stress, Psychological/*metabolism
MH  - Suprachiasmatic Nucleus/*physiology
OTO - NOTNLM
OT  - Adrenal gland
OT  - Period2
OT  - circadian clock
OT  - clock gene
OT  - social defeat
OT  - suprachiasmatic nucleus
EDAT- 2014/07/23 06:00
MHDA- 2015/08/19 06:00
CRDT- 2014/07/23 06:00
PHST- 2014/07/23 06:00 [entrez]
PHST- 2014/07/23 06:00 [pubmed]
PHST- 2015/08/19 06:00 [medline]
AID - 10.3109/07420528.2014.940085 [doi]
PST - ppublish
SO  - Chronobiol Int. 2014 Nov;31(9):996-1007. doi: 10.3109/07420528.2014.940085. Epub 
      2014 Jul 22.