PMID- 25051963
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20211021
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 124
IP  - 10
DP  - 2014 Sep 4
TI  - Gfi-1 regulates the erythroid transcription factor network through Id2 repression 
      in murine hematopoietic progenitor cells.
PG  - 1586-96
LID - 10.1182/blood-2014-02-556522 [doi]
AB  - Growth factor independence 1 (Gfi-1) is a part of the transcriptional network 
      that regulates the development of adult hematopoietic stem and progenitor cells. 
      Gfi-1-null (Gfi-1(-/-)) mice have reduced numbers of hematopoietic stem cells 
      (HSCs), impaired radioprotective function of hematopoietic progenitor cells 
      (HPCs), and myeloid and erythroid hyperplasia. We found that the development of 
      HPCs and erythropoiesis, but not HSC function, was rescued by reducing the 
      expression of inhibitor of DNA-binding protein 2 (Id2) in Gfi-1(-/-) mice. 
      Analysis of Gfi-1(-/-);Id2(+/-) mice revealed that short-term HSCs, common 
      myeloid progenitors (CMPs), erythroid burst-forming units, colony-forming units 
      in spleen, and more differentiated red cells were partially restored by reducing 
      Id2 levels in Gfi-1(-/-) mice. Moreover, short-term reconstituting cells, and, to 
      a greater extent, CMP and megakaryocyte-erythroid progenitor development, and red 
      blood cell production (anemia) were rescued in mice transplanted with 
      Gfi-1(-/-);Id2(+/-) bone marrow cells (BMCs) in comparison with Gfi-1(-/-) BMCs. 
      Reduction of Id2 expression in Gfi-1(-/-) mice increased the expression of Gata1, 
      Eklf, and EpoR, which are required for proper erythropoiesis. Reducing the levels 
      of other Id family members (Id1 and Id3) in Gfi-1(-/-) mice did not rescue 
      impaired HPC function or erythropoiesis. These data provide new evidence that 
      Gfi-1 is linked to the erythroid gene regulatory network by repressing Id2 
      expression.
FAU - Kim, Wonil
AU  - Kim W
AD  - Basic Science Program, Leidos Biomedical Research, Inc., Mouse Cancer and 
      Genetics Program, Frederick National Laboratory for Cancer Research, Frederick, 
      MD.
FAU - Klarmann, Kimberly D
AU  - Klarmann KD
AD  - Basic Science Program, Leidos Biomedical Research, Inc., Mouse Cancer and 
      Genetics Program, Frederick National Laboratory for Cancer Research, Frederick, 
      MD.
FAU - Keller, Jonathan R
AU  - Keller JR
AD  - Basic Science Program, Leidos Biomedical Research, Inc., Mouse Cancer and 
      Genetics Program, Frederick National Laboratory for Cancer Research, Frederick, 
      MD.
LA  - eng
GR  - HHSN261200800001C/RC/CCR NIH HHS/United States
GR  - HHSN261200800001E/CA/NCI NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
DEP - 20140722
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gfi1 protein, mouse)
RN  - 0 (Idb2 protein, mouse)
RN  - 0 (Inhibitor of Differentiation Protein 2)
RN  - 0 (Transcription Factors)
SB  - IM
EIN - Blood. 2016 Mar 17;127(11):1517
EIN - Blood. 2016 Mar 17;127(11):1517. PMID: 31265489
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*physiology
MH  - Down-Regulation/genetics
MH  - Erythroid Precursor Cells/physiology
MH  - Erythropoiesis/*genetics
MH  - *Gene Regulatory Networks
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Inhibitor of Differentiation Protein 2/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Transcription Factors/*genetics/metabolism/physiology
PMC - PMC4155270
EDAT- 2014/07/24 06:00
MHDA- 2014/11/19 06:00
PMCR- 2015/09/04
CRDT- 2014/07/24 06:00
PHST- 2014/07/24 06:00 [entrez]
PHST- 2014/07/24 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2015/09/04 00:00 [pmc-release]
AID - S0006-4971(20)39768-8 [pii]
AID - 2014/556522 [pii]
AID - 10.1182/blood-2014-02-556522 [doi]
PST - ppublish
SO  - Blood. 2014 Sep 4;124(10):1586-96. doi: 10.1182/blood-2014-02-556522. Epub 2014 
      Jul 22.