PMID- 25052363
OWN - NLM
STAT- MEDLINE
DCOM- 20141126
LR  - 20211021
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 178
IP  - 2
DP  - 2014 Nov
TI  - Are urinary levels of high mobility group box 1 markers of active nephritis in 
      anti-neutrophil cytoplasmic antibody-associated vasculitis?
PG  - 270-8
LID - 10.1111/cei.12422 [doi]
AB  - The objective of this study is to evaluate urinary high mobility group box 1 
      (HMGB1) levels as markers for active nephritis in patients with anti-neutrophil 
      cytoplasmic antibody (ANCA)-associated vasculitis (AAV) in comparison with 
      urinary CD4(+) effector memory T cells and urinary monocyte chemoattractant 
      protein-1 (MCP-1). Twenty-four AAV patients with active nephritis and 12 healthy 
      controls (HC) were evaluated. In nine patients, samples were also obtained during 
      remission. Urinary levels of HMGB1 were measured by Western blot. CD4(+) T cells 
      and CD4(+) effector memory T cells (CD4(+) CD45RO(+) CCR7(-) ) were determined in 
      urine and whole blood by flow cytometry. Measurement of urinary levels of MCP-1 
      and serum HMGB1 levels were performed by enzyme-linked immunosorbent assay 
      (ELISA). AAV patients with active nephritis had higher median intensity of HMGB1 
      in urine than HC [10.3 (7.05-18.50) versus 5.8 (4.48-7.01); P = 0.004]. Both 
      urinary HMGB1 and MCP-1 levels decreased significantly from active nephritis to 
      remission. The urinary MCP-1/creatinine ratio correlated with Birmingham 
      Vasculitis Activity Score (BVAS) (P = 0.042). No correlation was found between 
      the HMGB1/creatinine ratio and 24-h proteinuria, estimated glomerular filtration 
      rate (eGFR), MCP-1/creatinine ratio, BVAS and serum HMGB1. A positive correlation 
      was found between urinary HMGB1/creatinine ratio and CD4(+) T cells/creatinine 
      ratio (P = 0.028) and effector memory T cells/creatinine ratio (P = 0.039) in 
      urine. Urinary HMGB1 levels are increased in AAV patients with active nephritis 
      when compared with HC and patients in remission, and urinary HMGB1 levels are 
      associated with CD4(+) T cells and CD4(+) effector memory T cells in urine. 
      Measurement of urinary HMGB1 may be of additional value in identifying active 
      glomerulonephritis in AAV patients.
CI  - (c) 2014 British Society for Immunology.
FAU - de Souza, A W S
AU  - de Souza AW
AD  - Department of Rheumatology and Clinical Immunology, University Medical Center 
      Groningen, University of Groningen, Groningen, the Netherlands; Rheumatology 
      Division, Department of Internal Medicine, Universidade Federal de Sao 
      Paulo/Escola Paulista de Medicina (Unifesp/EPM), Sao Paulo, Brazil.
FAU - Abdulahad, W H
AU  - Abdulahad WH
FAU - Sosicka, P
AU  - Sosicka P
FAU - Bijzet, J
AU  - Bijzet J
FAU - Limburg, P C
AU  - Limburg PC
FAU - Stegeman, C A
AU  - Stegeman CA
FAU - Bijl, M
AU  - Bijl M
FAU - Westra, J
AU  - Westra J
FAU - Kallenberg, C G M
AU  - Kallenberg CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HMGB1 Protein)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anti-Neutrophil Cytoplasmic Antibody-Associated 
      Vasculitis/*complications/diagnosis/immunology
MH  - Biomarkers
MH  - Chemokine CCL2/urine
MH  - Female
MH  - Glomerulonephritis/blood/*etiology/*urine
MH  - HMGB1 Protein/blood/*urine
MH  - Humans
MH  - Immunologic Memory
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocyte Subsets/immunology/metabolism
PMC - PMC4233377
OTO - NOTNLM
OT  - HMGB1
OT  - T cells
OT  - anti-neutrophil cytoplasmic antibody-associated vasculitis
OT  - glomerulonephritis
OT  - monocyte chemoattractant protein-1
EDAT- 2014/07/24 06:00
MHDA- 2014/12/15 06:00
PMCR- 2015/11/01
CRDT- 2014/07/24 06:00
PHST- 2014/07/20 00:00 [accepted]
PHST- 2014/07/24 06:00 [entrez]
PHST- 2014/07/24 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - 10.1111/cei.12422 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2014 Nov;178(2):270-8. doi: 10.1111/cei.12422.