PMID- 25053052
OWN - NLM
STAT- MEDLINE
DCOM- 20151202
LR  - 20200812
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 166
DP  - 2015 Jan 1
TI  - IgE binding to peanut allergens is inhibited by combined D-aspartic and 
      D-glutamic acids.
PG  - 248-253
LID - S0308-8146(14)00890-5 [pii]
LID - 10.1016/j.foodchem.2014.06.004 [doi]
AB  - The objective of this study was to determine if D-amino acids (D-aas) bind and 
      inhibit immunoglobulin E (IgE) binding to peanut allergens. D-aas such as D-Asp 
      (aspartic acid), D-Glu (glutamic acid), combined D-[Asp/Glu] and others were each 
      prepared in a cocktail of 9 other D-aas, along with L-amino acids (L-aas) and 
      controls. Each sample was mixed with a pooled plasma from peanut-allergic donors, 
      and tested by ELISA (enzyme-linked immunosorbent assay) and Western blots for IgE 
      binding to peanut allergens. Results showed that D-[Asp/Glu] (4 mg/ml) inhibited 
      IgE binding (75%) while D-Glu, D-Asp and other D-aas had no inhibitory effect. A 
      higher inhibition was seen with D-[Asp/Glu] than with L-[Asp/Glu]. We concluded 
      that IgE was specific for D-[Asp/Glu], not D-Asp or D-Glu, and that D-[Asp/Glu] 
      was more reactive than was L-[Asp/Glu] in IgE inhibition. The finding indicates 
      that D-[Asp/Glu] may have the potential for removing IgE or reducing IgE binding 
      to peanut allergens in vitro.
CI  - Published by Elsevier Ltd.
FAU - Chung, Si-Yin
AU  - Chung SY
AD  - United States Department of Agriculture, Agricultural Research Services, Southern 
      Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA. 
      Electronic address: siyin.chung@ars.usda.gov.
FAU - Reed, Shawndrika
AU  - Reed S
AD  - United States Department of Agriculture, Agricultural Research Services, Southern 
      Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140611
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Allergens)
RN  - 0 (Glutamates)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Arachis/chemistry/*immunology
MH  - Aspartic Acid/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glutamates/*chemistry
MH  - Immunoglobulin E/*immunology
OTO - NOTNLM
OT  - Ara h 1 and Ara h 2
OT  - IgE antibodies
OT  - Peanut allergens
OT  - d-Amino acids
OT  - d-Aspartic acid
OT  - d-Glutamic acid
EDAT- 2014/07/24 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/07/24 06:00
PHST- 2013/12/25 00:00 [received]
PHST- 2014/05/05 00:00 [revised]
PHST- 2014/06/03 00:00 [accepted]
PHST- 2014/07/24 06:00 [entrez]
PHST- 2014/07/24 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0308-8146(14)00890-5 [pii]
AID - 10.1016/j.foodchem.2014.06.004 [doi]
PST - ppublish
SO  - Food Chem. 2015 Jan 1;166:248-253. doi: 10.1016/j.foodchem.2014.06.004. Epub 2014 
      Jun 11.