PMID- 25054276
OWN - NLM
STAT- MEDLINE
DCOM- 20150420
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 7
DP  - 2014
TI  - Differentiation of human umbilical cord mesenchymal stem cells into prostate-like 
      epithelial cells in vivo.
PG  - e102657
LID - 10.1371/journal.pone.0102657 [doi]
LID - e102657
AB  - Although human umbilical cord mesenchymal stem cells (hUC-MSCs) have been 
      identified as a new source of MSCs for potential application in regenerative 
      medicine, their full potential of differentiation has not been determined. In 
      particular, whether they have the capability to differentiate into epithelial 
      cells of endodermal origin such as the prostate epithelial cells is unknown. Here 
      we report that when hUC-MSCs were combined with rat urogenital sinus stromal 
      cells (rUGSSs) and transplanted into the renal capsule in vivo, they could 
      differentiate into prostate epithelial-like cells that could be verified by 
      prostate epithelial cell-specific markers including the prostate specific 
      antigen. The prostatic glandular structures formed in vivo displayed similar 
      cellular architecture with lumens and branching features as seen for a normal 
      prostate. In addition, the human origin of the hUC-MSCs was confirmed by 
      immunocytochemistry for human nuclear antigen. These findings together indicate 
      that hUC-MSCs have the capability to differentiate into epithelial-like cells 
      that are normally derived from the endoderm, implicating their potential 
      applications in tissue repair and regeneration of many endoderm-derived internal 
      organs.
FAU - Li, Wang
AU  - Li W
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Ye, Bo
AU  - Ye B
AD  - Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Cai, Xiao-Yan
AU  - Cai XY
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Lin, Jian-Hua
AU  - Lin JH
AD  - Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Gao, Wei-Qiang
AU  - Gao WQ
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem 
      Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong 
      University, Shanghai, China; School of Biomedical Engineering & Med-X Research 
      Institute, Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140723
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Keratin-8)
RN  - 0 (Receptors, Androgen)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Epithelial Cells/chemistry/*cytology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratin-8/analysis
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/chemistry/*cytology
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Pregnancy
MH  - Prostate/chemistry/*cytology
MH  - Rats, Sprague-Dawley
MH  - Receptors, Androgen/analysis
MH  - Transplantation, Heterologous
MH  - Umbilical Cord/*cytology
MH  - Urogenital System/cytology
PMC - PMC4108360
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/07/24 06:00
MHDA- 2015/04/22 06:00
PMCR- 2014/07/23
CRDT- 2014/07/24 06:00
PHST- 2013/11/23 00:00 [received]
PHST- 2014/06/21 00:00 [accepted]
PHST- 2014/07/24 06:00 [entrez]
PHST- 2014/07/24 06:00 [pubmed]
PHST- 2015/04/22 06:00 [medline]
PHST- 2014/07/23 00:00 [pmc-release]
AID - PONE-D-13-48709 [pii]
AID - 10.1371/journal.pone.0102657 [doi]
PST - epublish
SO  - PLoS One. 2014 Jul 23;9(7):e102657. doi: 10.1371/journal.pone.0102657. 
      eCollection 2014.