PMID- 25054409
OWN - NLM
STAT- MEDLINE
DCOM- 20150618
LR  - 20231213
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 27
IP  - 11
DP  - 2014 Nov
TI  - Agroinfiltration by cytokinin-producing Agrobacterium sp. strain GV3101 primes 
      defense responses in Nicotiana tabacum.
PG  - 1175-85
LID - 10.1094/MPMI-04-14-0114-R [doi]
AB  - Transient infiltrations in tobacco are commonly used in plant studies, but the 
      host response to different disarmed Agrobacterium strains is not fully 
      understood. The present study shows that pretreatment with disarmed Agrobacterium 
      tumefaciens GV3101 primes the defense response to subsequent infection by 
      Pseudomonas syringae in Nicotiana tabacum. The presence of a trans-zeatin 
      synthase (tzs) gene in strain GV3101 may be partly responsible for the priming 
      response, as the tzs-deficient Agrobacterium sp. strain LBA4404 only weakly 
      imparts such responses. Besides inducing the expression of defense-related genes 
      like PR-1 and NHL10, GV3101 pretreatment increased the expression of tobacco 
      mitogen-activated protein kinase (MAPK) pathway genes like MEK2, WIPK 
      (wound-induced protein kinase), and SIPK (salicylic acid-induced protein kinase). 
      Furthermore, the GV3101 strain showed a stronger effect than the LBA4404 strain 
      in activating phosphorylation of the tobacco MAPK, WIPK and SIPK, which 
      presumably prime the plant immune machinery. Lower doses of exogenously applied 
      cytokinins increased the activation of MAPK, while higher doses decreased the 
      activation, suggesting a balanced level of cytokinins is required to generate 
      defense response in planta. The current study serves as a cautionary warning for 
      plant researchers over the choice of Agrobacterium strains and their possible 
      consequences on subsequent pathogen-related studies.
FAU - Sheikh, Arsheed Hussain
AU  - Sheikh AH
FAU - Raghuram, Badmi
AU  - Raghuram B
FAU - Eschen-Lippold, Lennart
AU  - Eschen-Lippold L
FAU - Scheel, Dierk
AU  - Scheel D
FAU - Lee, Justin
AU  - Lee J
FAU - Sinha, Alok Krishna
AU  - Sinha AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - 0 (Cytokinins)
RN  - 0 (Plant Proteins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (SA-induced protein kinase)
RN  - O414PZ4LPZ (Salicylic Acid)
SB  - IM
MH  - Agrobacterium tumefaciens/*physiology
MH  - Cytokinins/*metabolism/pharmacology
MH  - Disease Resistance
MH  - *Gene Expression Regulation, Plant/drug effects
MH  - MAP Kinase Signaling System
MH  - Mitogen-Activated Protein Kinases
MH  - Plant Diseases/*immunology/microbiology
MH  - Plant Leaves/drug effects/genetics/immunology/microbiology
MH  - Plant Proteins/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Protoplasts
MH  - Pseudomonas syringae/physiology
MH  - Salicylic Acid/metabolism
MH  - Nicotiana/drug effects/genetics/immunology/*microbiology
EDAT- 2014/07/24 06:00
MHDA- 2015/06/19 06:00
CRDT- 2014/07/24 06:00
PHST- 2014/07/24 06:00 [entrez]
PHST- 2014/07/24 06:00 [pubmed]
PHST- 2015/06/19 06:00 [medline]
AID - 10.1094/MPMI-04-14-0114-R [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2014 Nov;27(11):1175-85. doi: 
      10.1094/MPMI-04-14-0114-R.