PMID- 25055776
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140724
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1179
DP  - 2014
TI  - Random insertional-deletional strand exchange mutagenesis (RAISE): a simple 
      method for generating random insertion and deletion mutations.
PG  - 151-8
LID - 10.1007/978-1-4939-1053-3_10 [doi]
AB  - Although proteins can be artificially improved by random insertion and deletion 
      mutagenesis methods, these procedures are technically difficult. Here we describe 
      a simple method called random insertional-deletional strand exchange mutagenesis 
      (RAISE). This method is based on gene shuffling and can be used to introduce a 
      wide variety of insertions, deletions, and substitutions. RAISE involves three 
      steps: DNA fragmentation, attachment of a random short sequence, and 
      reconstruction. This yields unique mutants and can be a powerful technique for 
      protein engineering.
FAU - Fujii, Ryota
AU  - Fujii R
AD  - Synthetic Chemicals Laboratory, Mitsui Chemicals, Inc., 580-32 Nagaura, 
      Sodegaura, Chiba, 299-0265, Japan.
FAU - Kitaoka, Motomitsu
AU  - Kitaoka M
FAU - Hayashi, Kiyoshi
AU  - Hayashi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Mutagenesis, Insertional/*methods
MH  - Sequence Deletion/genetics
EDAT- 2014/07/25 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/07/25 06:00
PHST- 2014/07/25 06:00 [entrez]
PHST- 2014/07/25 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 10.1007/978-1-4939-1053-3_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1179:151-8. doi: 10.1007/978-1-4939-1053-3_10.