PMID- 25056831
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20211021
IS  - 1615-6102 (Electronic)
IS  - 0033-183X (Print)
IS  - 0033-183X (Linking)
VI  - 252
IP  - 1
DP  - 2015 Jan
TI  - Karyotype structure and chromosome fragility in the grass Phleum echinatum Host.
PG  - 301-6
LID - 10.1007/s00709-014-0681-5 [doi]
AB  - Phleum echinatum Host (2n = 2x = 10) is an annual Mediterranean species which 
      differs from other representatives of the genus Phleum by reduced chromosome 
      number, asymmetric karyotype and unusually high amount of DNA in the genome. 
      Chromosomes of this plant were studied using conventional acetic-orcein staining 
      and fluorescence in situ hybridization (FISH). FISH showed the major 35S 
      ribosomal DNA (rDNA) site at the secondary constriction of satellite chromosome 
      (3) and the minor 35S rDNA site near 5S rDNA cluster in the monobrachial 
      chromosome 5. Telomeric repeats were detected at all chromosome ends within 
      secondary constriction in satellited chromosome 3 and at the centromeric regions 
      of chromosomes 1 and 2. Intrachromosomally located telomeric repeats are probably 
      traces of chromosomal rearrangements that have shaped P.echinatum genome; they 
      were prone to breakage which was manifested in chromosome fragmentation. The most 
      distinct telomeric signals, suggesting massive amplification of interstitial 
      telomeric sequences (ITRs), were observed at the nucleolar organizer region (NOR) 
      of the third chromosome pair. Double FISH confirmed co-localization of telomeric 
      and 35S rDNA repeats in this locus characterized by the biggest fragility in the 
      karyotype. Fragile sites of P.echinatum, composed of amplified telomeric repeats, 
      may bear a resemblance to metazoan rare fragile sites enriched in microsatellite 
      repeats.
FAU - Grabowska-Joachimiak, Aleksandra
AU  - Grabowska-Joachimiak A
AD  - Department of Plant Breeding and Seed Science, University of Agriculture in 
      Cracow, Lobzowska 24, Cracow, 31-140, Poland, rrjoachi@cyf-kr.edu.pl.
FAU - Kula, Adam
AU  - Kula A
FAU - Gernand-Kliefoth, Dorota
AU  - Gernand-Kliefoth D
FAU - Joachimiak, Andrzej J
AU  - Joachimiak AJ
LA  - eng
PT  - Journal Article
DEP - 20140724
PL  - Austria
TA  - Protoplasma
JT  - Protoplasma
JID - 9806853
SB  - IM
MH  - Chromosome Banding/*methods
MH  - Chromosome Fragility/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Phleum/*chemistry
PMC - PMC4287660
EDAT- 2014/07/25 06:00
MHDA- 2016/02/11 06:00
PMCR- 2014/07/24
CRDT- 2014/07/25 06:00
PHST- 2014/06/05 00:00 [received]
PHST- 2014/07/14 00:00 [accepted]
PHST- 2014/07/25 06:00 [entrez]
PHST- 2014/07/25 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
PHST- 2014/07/24 00:00 [pmc-release]
AID - 681 [pii]
AID - 10.1007/s00709-014-0681-5 [doi]
PST - ppublish
SO  - Protoplasma. 2015 Jan;252(1):301-6. doi: 10.1007/s00709-014-0681-5. Epub 2014 Jul 
      24.