PMID- 25058511
OWN - NLM
STAT- MEDLINE
DCOM- 20150601
LR  - 20140923
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Linking)
VI  - 44
IP  - 11
DP  - 2014 Oct 1
TI  - Hc-fau, a novel gene regulating diapause in the nematode parasite Haemonchus 
      contortus.
PG  - 775-86
LID - S0020-7519(14)00165-9 [pii]
LID - 10.1016/j.ijpara.2014.05.011 [doi]
AB  - Diapause induced in the early fourth stage of Haemonchus contortus is a strategy 
      to adapt this nematode to hostile environmental conditions. In this study, we 
      identified a new gene, Hc-fau, a homologue of human fau and Caenorhabditis 
      elegans Ce-rps30. Hc-fau encodes two proteins through alternative RNA splicing, 
      Hc-FAUA and Hc-FAUB, consisting of 130 and 107 amino acids, respectively. Hc-FAU 
      possesses a diverged ubiquitin-like (UBiL) protein domain and a conserved 
      ribosome protein S30 domain. The protein is ubiquitously expressed, except in the 
      gonad. However Hc-fau transcripts decrease significantly in diapausing L4s of H. 
      contortus. In C. elegans, knockdown of Ce-rps30 confers an extended lifespan, 
      increased lipid storage in the intestine and shortened body length. These 
      morphological characteristics are comparable with dauer larvae of C. elegans, in 
      which the gonad is condensed considerably. In contrast, a shortened lifespan is 
      observed in C. elegans over-expressing Hc-faua, and especially Hc-faub, with 
      hatching failure detected. The genes of insulin/IGF-1 signalling (IIS), TGF-beta, 
      cGMP, dafachronic acid (DA), apoptosis (AP) and fatty acids (FA) metabolism are 
      all down-regulated in Ce-rps30RNAi (RNA interference) worms, except for akt-1 and 
      daf-16. However, daf-16 up-regulation is inconsistent with its target gene 
      down-regulation and the result from a heat stress assay in these worms. Daf-16 
      RNAi conducted in Ce-rps30 (tm6034/nt1) mutants failed to rescue the worms. The 
      S30 domain stays in the nucleus, while UBiL accumulates in the cytoplasm. 
      Compared with Hc-FAUA, results of UBiL domain and S30 domain over-expression 
      indicate synergism between UBiL and S30 in regulating lifespan and reproduction. 
      These results suggest the potential functions of Hc-fau in regulating larval 
      diapause in H.contortus.
CI  - Copyright (c) 2014. Published by Elsevier Ltd.
FAU - Yan, Baolong
AU  - Yan B
AD  - Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory 
      of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
FAU - Guo, Xiaolu
AU  - Guo X
AD  - Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory 
      of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
FAU - Zhou, Qianjin
AU  - Zhou Q
AD  - School of Marine Science, Ningbo University, Ningbo 315211, China.
FAU - Yang, Yi
AU  - Yang Y
AD  - Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory 
      of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
FAU - Chen, Xueqiu
AU  - Chen X
AD  - Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory 
      of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
FAU - Sun, Weiwei
AU  - Sun W
AD  - Laboratory for Evolution & Development, Institute of Evolution & Marine 
      Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 
      266003, China.
FAU - Du, Aifang
AU  - Du A
AD  - Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory 
      of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China. 
      Electronic address: afdu@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140721
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Helminth Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caenorhabditis elegans
MH  - Caenorhabditis elegans Proteins/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Gene Expression Regulation/physiology
MH  - Haemonchus/*genetics/growth & development/*physiology
MH  - Helminth Proteins/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - Caenorhabditis elegans
OT  - Diapause
OT  - Haemonchus contortus
OT  - Hc-fau
OT  - Nematode
EDAT- 2014/07/25 06:00
MHDA- 2015/06/02 06:00
CRDT- 2014/07/25 06:00
PHST- 2014/03/06 00:00 [received]
PHST- 2014/05/27 00:00 [revised]
PHST- 2014/05/28 00:00 [accepted]
PHST- 2014/07/25 06:00 [entrez]
PHST- 2014/07/25 06:00 [pubmed]
PHST- 2015/06/02 06:00 [medline]
AID - S0020-7519(14)00165-9 [pii]
AID - 10.1016/j.ijpara.2014.05.011 [doi]
PST - ppublish
SO  - Int J Parasitol. 2014 Oct 1;44(11):775-86. doi: 10.1016/j.ijpara.2014.05.011. 
      Epub 2014 Jul 21.