PMID- 25059794
OWN - NLM
STAT- MEDLINE
DCOM- 20150916
LR  - 20220309
IS  - 1932-846X (Electronic)
IS  - 1932-8451 (Print)
IS  - 1932-8451 (Linking)
VI  - 75
IP  - 2
DP  - 2015 Feb
TI  - Withdrawal of BDNF from hippocampal cultures leads to changes in genes involved 
      in synaptic function.
PG  - 173-92
LID - 10.1002/dneu.22216 [doi]
AB  - Neurotrophins play a crucial role in mediating neuronal survival and synaptic 
      plasticity. A lack of trophic factor support in the peripheral nervous system 
      (PNS) is associated with a transcription-dependent programmed cell death process 
      in developing sympathetic neurons. While most of the attention has been on events 
      culminating in cell death in the PNS, the earliest events that occur after 
      trophic factor withdrawal in the central nervous system (CNS) have not been 
      investigated. In the CNS, brain-derived neurotrophic factor (BDNF) is widely 
      expressed and is released in an activity-dependent manner to shape the structure 
      and function of neuronal populations. Reduced neurotrophic factor support has 
      been proposed as a mechanism to account for changes in synaptic plasticity during 
      neurodevelopment to aging and neurodegenerative disorders. To this end, we 
      performed transcriptional profiling in cultured rat hippocampal neurons. We used 
      a TrkB ligand scavenger (TrkB-FC ) to sequester endogenous neurotrophic factor 
      activity from hippocampal neurons in culture. Using a high-density microarray 
      platform, we identified a significant decrease in genes that are associated with 
      vesicular trafficking and synaptic function, as well as selective increases in 
      MAP kinase phosphatases. A comparison of these changes with recent studies of 
      Alzheimer's disease and cognitive impairment in postmortem brain tissue revealed 
      striking similarities in gene expression changes for genes involved in synaptic 
      function. These changes are relevant to a wide number of conditions in which 
      levels of BDNF are compromised.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Mariga, Abigail
AU  - Mariga A
AD  - Cell and Molecular Biology Program, New York University Langone Medical Center, 
      New York, New York, 10016.
FAU - Zavadil, Jiri
AU  - Zavadil J
FAU - Ginsberg, Stephen D
AU  - Ginsberg SD
FAU - Chao, Moses V
AU  - Chao MV
LA  - eng
GR  - P30 CA016087/CA/NCI NIH HHS/United States
GR  - NS21072/NS/NINDS NIH HHS/United States
GR  - AG025970/AG/NIA NIH HHS/United States
GR  - P01 AG014449/AG/NIA NIH HHS/United States
GR  - AG043375/AG/NIA NIH HHS/United States
GR  - R01 AG025970/AG/NIA NIH HHS/United States
GR  - P01 AG017617/AG/NIA NIH HHS/United States
GR  - R56 NS021072/NS/NINDS NIH HHS/United States
GR  - AG014449/AG/NIA NIH HHS/United States
GR  - AG017617/AG/NIA NIH HHS/United States
GR  - R01 AG043375/AG/NIA NIH HHS/United States
GR  - R01 NS021072/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140825
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Central Nervous System Agents)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.3.16 (Mitogen-Activated Protein Kinase Phosphatases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*deficiency
MH  - Cells, Cultured
MH  - Central Nervous System Agents/pharmacology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/physiology
MH  - Hippocampus/drug effects/*physiology
MH  - Microarray Analysis
MH  - Mitogen-Activated Protein Kinase Phosphatases/genetics/metabolism
MH  - Neuronal Plasticity/drug effects/genetics/*physiology
MH  - Neurons/drug effects/*physiology
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, trkB/agonists
PMC - PMC4329925
MID - NIHMS660972
OTO - NOTNLM
OT  - BDNF deprivation
OT  - hippocampus
OT  - microarray
OT  - neurodegeneration
OT  - synaptic function
OT  - transcription
EDAT- 2014/07/26 06:00
MHDA- 2015/09/17 06:00
PMCR- 2016/02/01
CRDT- 2014/07/26 06:00
PHST- 2014/01/14 00:00 [received]
PHST- 2014/06/26 00:00 [revised]
PHST- 2014/07/23 00:00 [accepted]
PHST- 2014/07/26 06:00 [entrez]
PHST- 2014/07/26 06:00 [pubmed]
PHST- 2015/09/17 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - 10.1002/dneu.22216 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2015 Feb;75(2):173-92. doi: 10.1002/dneu.22216. Epub 2014 Aug 25.