PMID- 25064513
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20211021
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 159
IP  - 11
DP  - 2014 Nov
TI  - Chimeric influenza-virus-like particles containing the porcine reproductive and 
      respiratory syndrome virus GP5 protein and the influenza virus HA and M1 
      proteins.
PG  - 3043-51
LID - 10.1007/s00705-014-2178-0 [doi]
AB  - Both porcine reproductive and respiratory syndrome and swine influenza are acute, 
      highly contagious swine diseases. These diseases pose severe threats for the 
      swine industry and cause heavy economic losses worldwide. In this study, we have 
      developed a chimeric virus-like particle (VLP) vaccine candidate for porcine 
      reproductive and respiratory syndrome virus (PRRSV) and H3N2 influenza virus and 
      investigated its immunogenicity in mice. The HA and M1 proteins from the H3N2 
      influenza virus and the PRRSV GP5 protein fused to the cytoplasmic and 
      transmembrane domains of the NA protein were both incorporated into the chimeric 
      VLPs. Analysis of the immune responses showed that the chimeric VLPs elicited 
      serum antibodies specific for both PRRSV GP5 and the H3N2 HA protein, and they 
      stimulated cellular immune responses compared to the responses to equivalent 
      amounts of inactivated viruses. Taken together, the results suggested that the 
      chimeric VLP vaccine represents a potential strategy for the development of a 
      safe and effective vaccine to control PRRSV and H3N2 influenza virus.
FAU - Xue, Chunyi
AU  - Xue C
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510006, People's Republic of China.
FAU - Wang, Wei
AU  - Wang W
FAU - Liu, Qiliang
AU  - Liu Q
FAU - Miao, Zhongwei
AU  - Miao Z
FAU - Liu, Kang
AU  - Liu K
FAU - Shen, Huifang
AU  - Shen H
FAU - Lv, Lishan
AU  - Lv L
FAU - Li, Xiaoming
AU  - Li X
FAU - Chen, Xiaochun
AU  - Chen X
FAU - Cao, Yongchang
AU  - Cao Y
LA  - eng
PT  - Journal Article
DEP - 20140727
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (Hemagglutinins, Viral)
RN  - 0 (M1 protein, Influenza A virus)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Female
MH  - Hemagglutinins, Viral/administration & dosage/genetics/*immunology
MH  - Influenza A Virus, H3N2 Subtype/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Orthomyxoviridae Infections/immunology/prevention & control/*veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Recombinant Fusion Proteins/administration & dosage/genetics/immunology
MH  - Swine
MH  - Swine Diseases/immunology/*prevention & control/virology
MH  - Viral Envelope Proteins/administration & dosage/genetics/*immunology
MH  - Viral Matrix Proteins/administration & dosage/genetics/*immunology
MH  - Viral Vaccines/administration & dosage/genetics/immunology
PMC - PMC7086999
EDAT- 2014/07/30 06:00
MHDA- 2014/12/23 06:00
PMCR- 2020/03/23
CRDT- 2014/07/28 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2014/07/11 00:00 [accepted]
PHST- 2014/07/28 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 2178 [pii]
AID - 10.1007/s00705-014-2178-0 [doi]
PST - ppublish
SO  - Arch Virol. 2014 Nov;159(11):3043-51. doi: 10.1007/s00705-014-2178-0. Epub 2014 
      Jul 27.