PMID- 25064634
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20221207
IS  - 1615-6102 (Electronic)
IS  - 0033-183X (Print)
IS  - 0033-183X (Linking)
VI  - 252
IP  - 1
DP  - 2015 Jan
TI  - Reactive oxygen and nitrogen (ROS and RNS) species generation and cell death in 
      tomato suspension cultures--Botrytis cinerea interaction.
PG  - 307-19
LID - 10.1007/s00709-014-0680-6 [doi]
AB  - This article reports events connected to cell survival and Botrytis cinerea 
      infection development in cell suspension cultures of two tomato cultivars which 
      show different levels of susceptibility to the pathogen: cv. Corindo (more 
      susceptible) and cv. Perkoz (less susceptible). In parallel changes in reactive 
      oxygen (ROS) and nitrogen (RNS) species generation and in S-nitrosoglutathione 
      reductase (GSNOR) activity were studied. In vivo staining methods with acridine 
      orange (AO) and ethidium bromide (EB) as well as fluorescent microscopy were used 
      to assess tomato and B. cinerea cells death. The biochemical studies of ROS and 
      RNS concentrations in plant cell extract were complemented by in vivo ROS and 
      nitric oxide (NO) imaging using nitro blue tetrazolium (NBT), diaminobenzidine 
      (DAB) and diaminofluorescein diacetate (DAF-DA) staining methods, and confocal 
      microscope technique. B. cinerea infection proceeded slower in Perkoz cell 
      cultures. It was evidenced by measuring the pathogen conidia germination and 
      germination tube development in which nuclei revealing cell death dominated. Two 
      different types of tomato cell death were observed: cells with necrotic nuclei 
      dominated in Corindo whereas in Perkoz cells with characteristic of vacuolar 
      death type prevailed. In Perkoz cells, constitutive levels of NO and 
      S-nitrosothiols (SNO) were significantly higher and hydrogen peroxide (H(2)O(2)) and 
      superoxide anion (O(2)(-)) concentrations were slightly higher as compared with 
      Corindo cells. Moreover, increases in these molecule concentrations as a result 
      of B. cinerea inoculation were observed in both, Perkoz and Corindo cell 
      cultures. The enzymatic GSNOR activity seems to be an important player in 
      controlling the SNO level in tomato cells. Involvements of the studied compounds 
      in molecular mechanisms of tomato resistance to B. cinerea are discussed in the 
      paper.
FAU - Pietrowska, E
AU  - Pietrowska E
AD  - Department of Plant Physiology and Biochemistry, University of Lodz, Banacha 
      12/16, 90-237, Lodz, Poland.
FAU - Rozalska, S
AU  - Rozalska S
FAU - Kazmierczak, A
AU  - Kazmierczak A
FAU - Nawrocka, J
AU  - Nawrocka J
FAU - Malolepsza, U
AU  - Malolepsza U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140727
PL  - Austria
TA  - Protoplasma
JT  - Protoplasma
JID - 9806853
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Botrytis
MH  - Cell Death
MH  - Solanum lycopersicum/*chemistry
MH  - Nitric Oxide/*metabolism
MH  - Reactive Oxygen Species/*metabolism
PMC - PMC4287684
EDAT- 2014/07/30 06:00
MHDA- 2016/02/11 06:00
PMCR- 2014/07/27
CRDT- 2014/07/28 06:00
PHST- 2014/04/29 00:00 [received]
PHST- 2014/07/12 00:00 [accepted]
PHST- 2014/07/28 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
PHST- 2014/07/27 00:00 [pmc-release]
AID - 680 [pii]
AID - 10.1007/s00709-014-0680-6 [doi]
PST - ppublish
SO  - Protoplasma. 2015 Jan;252(1):307-19. doi: 10.1007/s00709-014-0680-6. Epub 2014 
      Jul 27.