PMID- 25069776
OWN - NLM
STAT- MEDLINE
DCOM- 20160106
LR  - 20211021
IS  - 2157-6564 (Print)
IS  - 2157-6580 (Electronic)
IS  - 2157-6564 (Linking)
VI  - 3
IP  - 9
DP  - 2014 Sep
TI  - Overexpression of polysialylated neural cell adhesion molecule improves the 
      migration capacity of induced pluripotent stem cell-derived oligodendrocyte 
      precursors.
PG  - 1100-9
LID - 10.5966/sctm.2014-0041 [doi]
AB  - Cell replacement therapy aiming at the compensation of lost oligodendrocytes and 
      restoration of myelination in acquired or congenital demyelination disorders has 
      gained considerable interest since the discovery of induced pluripotent stem 
      cells (iPSCs). Patient-derived iPSCs provide an inexhaustible source for 
      transplantable autologous oligodendrocyte precursors (OPCs). The first 
      transplantation studies in animal models for demyelination with iPSC-derived OPCs 
      demonstrated their survival and remyelinating capacity, but also revealed their 
      limited migration capacity. In the present study, we induced overexpression of 
      the polysialylating enzyme sialyltransferase X (STX) in iPSC-derived OPCs to 
      stimulate the production of polysialic acid-neuronal cell adhesion molecules 
      (PSA-NCAMs), known to promote and facilitate the migration of OPCs. The 
      STX-overexpressing iPSC-derived OPCs showed a normal differentiation and 
      maturation pattern and were able to downregulate PSA-NCAMs when they became 
      myelin-forming oligodendrocytes. After implantation in the demyelinated corpus 
      callosum of cuprizone-fed mice, STX-expressing iPSC-derived OPCs demonstrated a 
      significant increase in migration along the axons. Our findings suggest that the 
      reach and efficacy of iPSC-derived OPC transplantation can be improved by 
      stimulating the OPC migration potential via specific gene modulation.
CI  - (c)AlphaMed Press.
FAU - Czepiel, Marcin
AU  - Czepiel M
AD  - Department of Neuroscience, University Medical Centre Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Leicher, Lasse
AU  - Leicher L
AD  - Department of Neuroscience, University Medical Centre Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Becker, Katja
AU  - Becker K
AD  - Department of Neuroscience, University Medical Centre Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Boddeke, Erik
AU  - Boddeke E
AD  - Department of Neuroscience, University Medical Centre Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Copray, Sjef
AU  - Copray S
AD  - Department of Neuroscience, University Medical Centre Groningen, University of 
      Groningen, Groningen, The Netherlands j.c.v.m.copray@umcg.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140728
PL  - England
TA  - Stem Cells Transl Med
JT  - Stem cells translational medicine
JID - 101578022
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - EC 2.4.99.- (CMP-N-acetylneuraminate-poly-alpha-2,8-sialosyl sialyltransferase)
RN  - EC 2.4.99.- (Sialyltransferases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/physiology
MH  - Cell Movement/*physiology
MH  - Coculture Techniques
MH  - Demyelinating Diseases/pathology
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Induced Pluripotent Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Cell Adhesion Molecules/*biosynthesis
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Oligodendroglia/*cytology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sialyltransferases/genetics/metabolism
MH  - Stem Cell Transplantation/methods
MH  - Transfection
PMC - PMC4149301
OTO - NOTNLM
OT  - Cell transplantation
OT  - Differentiation
OT  - Oligodendrocyte
OT  - Pluripotent stem cells
OT  - Remyelination
EDAT- 2014/07/30 06:00
MHDA- 2016/01/07 06:00
PMCR- 2015/09/01
CRDT- 2014/07/30 06:00
PHST- 2014/07/30 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2016/01/07 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - sctm.2014-0041 [pii]
AID - 20140041 [pii]
AID - 10.5966/sctm.2014-0041 [doi]
PST - ppublish
SO  - Stem Cells Transl Med. 2014 Sep;3(9):1100-9. doi: 10.5966/sctm.2014-0041. Epub 
      2014 Jul 28.