PMID- 25071430
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140729
LR  - 20211021
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 8
DP  - 2014
TI  - Electrophysiological characterization of male goldfish (Carassius auratus) 
      ventral preoptic area neurons receiving olfactory inputs.
PG  - 185
LID - 10.3389/fnins.2014.00185 [doi]
LID - 185
AB  - Chemical communication via sex pheromones is critical for successful reproduction 
      but the underlying neural mechanisms are not well-understood. The goldfish is a 
      tractable model because sex pheromones have been well-characterized in this 
      species. We used male goldfish forebrain explants in vitro and performed 
      whole-cell current clamp recordings from single neurons in the ventral preoptic 
      area (vPOA) to characterize their membrane properties and synaptic inputs from 
      the olfactory bulbs (OB). Principle component and cluster analyses based on 
      intrinsic membrane properties of vPOA neurons (N = 107) revealed five (I-V) 
      distinct cell groups. These cells displayed differences in their input resistance 
      (Rinput: I < II < IV < III = V), time constant (TC: I = II < IV < III = V), and 
      threshold current (Ithreshold: I > II = IV > III = V). Evidence from electrical 
      stimulation of the OB and application of receptor antagonists suggests that vPOA 
      neurons receive monosynaptic glutamatergic inputs via the medial olfactory tract, 
      with connectivity varying among neuronal groups [I (24%), II (40%), III (0%), IV 
      (34%), and V (2%)].
FAU - Lado, Wudu E
AU  - Lado WE
AD  - Department of Biology, University of Ottawa Ottawa, ON, Canada ; Department of 
      Cell and Systems Biology, University of Toronto Toronto, ON, Canada.
FAU - Spanswick, David C
AU  - Spanswick DC
AD  - Warwick Medical School, University of Warwick Coventry, UK ; Department of 
      Physiology, Monash University Clayton, VIC, Australia.
FAU - Lewis, John E
AU  - Lewis JE
AD  - Department of Biology, University of Ottawa Ottawa, ON, Canada.
FAU - Trudeau, Vance L
AU  - Trudeau VL
AD  - Department of Biology, University of Ottawa Ottawa, ON, Canada.
LA  - eng
PT  - Journal Article
DEP - 20140630
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC4074913
OTO - NOTNLM
OT  - male goldfish
OT  - monosynaptic glutamatergic projections
OT  - ventral preoptic area
EDAT- 2014/07/30 06:00
MHDA- 2014/07/30 06:01
PMCR- 2014/01/01
CRDT- 2014/07/30 06:00
PHST- 2014/03/31 00:00 [received]
PHST- 2014/06/11 00:00 [accepted]
PHST- 2014/07/30 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2014/07/30 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2014.00185 [doi]
PST - epublish
SO  - Front Neurosci. 2014 Jun 30;8:185. doi: 10.3389/fnins.2014.00185. eCollection 
      2014.