PMID- 25072247
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 7
DP  - 2014
TI  - Effects of a honeybee sting on the serum free amino acid profile in humans.
PG  - e103533
LID - 10.1371/journal.pone.0103533 [doi]
LID - e103533
AB  - The aim of this study was to assess the response to a honeybee venom by analyzing 
      serum levels of 34 free amino acids. Another goal of this study was to apply 
      complex analytic-bioinformatic-clinical strategy based on up-to-date achievements 
      of mass spectrometry in metabolomic profiling. The amino acid profiles were 
      determined using hybrid triple quadrupole/linear ion trap mass spectrometer 
      coupled with a liquid chromatography instrument. Serum samples were collected 
      from 27 beekeepers within 3 hours after they were stung and after a minimum of 6 
      weeks following the last sting. The differences in amino acid profiles were 
      evaluated using MetaboAnalyst and ROCCET web portals. Chemometric tests showed 
      statistically significant differences in the levels of L-glutamine (Gln), 
      L-glutamic acid (Glu), L-methionine (Met) and 3-methyl-L-histidine (3MHis) 
      between the two analyzed groups of serum samples. Gln and Glu appeared to be the 
      most important metabolites for distinguishing the beekeepers tested shortly after 
      a bee sting from those tested at least 6 weeks later. The role of some amino 
      acids in the response of an organism to the honeybee sting was also discussed. 
      This study indicated that proposed methodology may allow to identify the 
      individuals just after the sting and those who were stung at least 6 weeks 
      earlier. The results we obtained will contribute to better understanding of the 
      human body response to the honeybee sting.
FAU - Matysiak, Jan
AU  - Matysiak J
AD  - Department of Inorganic & Analytical Chemistry, Poznan University of Medical 
      Sciences, Poznan, Poland.
FAU - Derezinski, Pawel
AU  - Derezinski P
AD  - Department of Inorganic & Analytical Chemistry, Poznan University of Medical 
      Sciences, Poznan, Poland.
FAU - Klupczynska, Agnieszka
AU  - Klupczynska A
AD  - Department of Inorganic & Analytical Chemistry, Poznan University of Medical 
      Sciences, Poznan, Poland.
FAU - Matysiak, Joanna
AU  - Matysiak J
AD  - Ward of Paediatric Diseases, L. Perzyna Regional Unified Hospital in Kalisz, 
      Kalisz, Poland.
FAU - Kaczmarek, Elzbieta
AU  - Kaczmarek E
AD  - Department of Bioinformatics and Computational Biology, Poznan University of 
      Medical Sciences, Poznan, Poland.
FAU - Kokot, Zenon J
AU  - Kokot ZJ
AD  - Department of Inorganic & Analytical Chemistry, Poznan University of Medical 
      Sciences, Poznan, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140729
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Amino Acids)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acids/*blood/metabolism
MH  - Animals
MH  - Area Under Curve
MH  - Beekeeping
MH  - Bees/physiology
MH  - *Bites and Stings
MH  - *Chromatography, High Pressure Liquid
MH  - Discriminant Analysis
MH  - Female
MH  - Humans
MH  - Least-Squares Analysis
MH  - Male
MH  - Middle Aged
MH  - ROC Curve
MH  - *Spectrometry, Mass, Electrospray Ionization
PMC - PMC4114776
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/07/30 06:00
MHDA- 2015/12/15 06:00
PMCR- 2014/07/29
CRDT- 2014/07/30 06:00
PHST- 2014/04/22 00:00 [received]
PHST- 2014/06/23 00:00 [accepted]
PHST- 2014/07/30 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2014/07/29 00:00 [pmc-release]
AID - PONE-D-14-18156 [pii]
AID - 10.1371/journal.pone.0103533 [doi]
PST - epublish
SO  - PLoS One. 2014 Jul 29;9(7):e103533. doi: 10.1371/journal.pone.0103533. 
      eCollection 2014.