PMID- 25072723
OWN - NLM
STAT- MEDLINE
DCOM- 20140922
LR  - 20140730
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 77
IP  - 14-16
DP  - 2014
TI  - Effects of naproxen on cell proliferation and genotoxicity in MG-63 osteosarcoma 
      cell line.
PG  - 916-23
LID - 10.1080/15287394.2014.911131 [doi]
AB  - The purpose of this study was to determine the efficacy of naproxen, a 
      nonsteroidal anti-inflammatory drug, on the MG-63 human osteosarcoma cell line. 
      MG-63 cells were exposed to naproxen in a wide range of concentrations of 0.03, 
      0.05, 0.1, 0.42, 0.83, and 1.67 mg/ml for 72 h. The activity of naproxen was 
      assessed by the following assays: cell morphology; 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method; 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay; comet assay; and acridine orange and monodansylcadaverine (MDC) staining. 
      Naproxen exerted a significant inhibitory effect on MG-63 cell proliferation, in 
      a concentration-dependent manner, in all treatment groups compared with untreated 
      cells. An increase in frequency of DNA damage, apoptotic bodies, apoptotic cells, 
      and autophagic vacuoles was observed in MG-63-treated cells. Although future 
      studies are needed, these findings suggest that naproxen may lead to improvements 
      in treatment of patients with osteosarcoma.
FAU - Correia, Isabel
AU  - Correia I
AD  - a Department of Genetics and Biotechnology and CECAV , University of 
      Tras-os-Montes and Alto Douro , Vila Real , Portugal.
FAU - Arantes-Rodrigues, Regina
AU  - Arantes-Rodrigues R
FAU - Pinto-Leite, Rosario
AU  - Pinto-Leite R
FAU - Gaivao, Isabel
AU  - Gaivao I
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 57Y76R9ATQ (Naproxen)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Comet Assay
MH  - DNA Damage/*drug effects
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Naproxen/*pharmacology
MH  - Osteosarcoma/pathology
EDAT- 2014/07/30 06:00
MHDA- 2014/09/23 06:00
CRDT- 2014/07/30 06:00
PHST- 2014/07/30 06:00 [entrez]
PHST- 2014/07/30 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - 10.1080/15287394.2014.911131 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2014;77(14-16):916-23. doi: 
      10.1080/15287394.2014.911131.