PMID- 25073286
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 28
IP  - 5
DP  - 2014 May
TI  - [Differentiation of bone marrow mesenchymal stem cells into insulin producing 
      cells induced by rat injured pancreatic tissue extract].
PG  - 625-32
AB  - OBJECTIVE: To investigate the effects and mechanisms of differentiation of bone 
      marrow mesenchymal stem cells (BMSCs) into insulin producing cells (IPCs) induced 
      by injured pancreatic tissue extract of rat. METHODS: Eighty 6-week-old Sprague 
      Dawley rats were selected. Forty rats underwent removal of 60% pancreas and the 
      injured pancreas tissue was obtained after 48 hours to prepare the injured 
      pancreatic tissue extract; and normal pancreatic tissue extract was prepared from 
      the other 40 rats. The BMSCs were isolated from the tibia and femur of 4-week-old 
      Sprague Dawley rats. BMSCs at passage 3 were co-cultured with rat injured 
      pancreatic tissue extract as experimental group, with rat normal pancreatic 
      tissue extract as normal control group, and with cell culture medium as blank 
      control group for 14 days. The expressions of pancreas development related genes 
      and proteins were detected, and cell morphological changes were observed. Then 
      the C peptide positive cell rate was detected by flow cytometry analysis and 
      insulin secretion levels were detected by glucose stimulation experiment at 14 
      days. RESULTS: Injured pancreatic tissue extract can induced BMSCs 
      differentiating into IPCs. The pancreatic development related genes of pancreatic 
      duodenal homeobox 1 (PDX-1), islet 1, Nkx6.1, glucose transporter type 2, 
      proprotein convertase 2, neurogenin 3, and somatostatin were expressed 
      sequentially in the differentiation process of experimental group; mature 
      pancreatic proteins of PDX-1, insulin, C peptide, Nkx6.1 also were expressed. But 
      there was no morphological changes and expression of pancreatic development 
      related genes and proteins in normal control and blank control groups. The C 
      peptide positive cell rate of experimental group (13.8% +/- 1.8%) were 
      significantly higher than those of normal control and blank control groups (1.6% 
      +/- 0.4%) (P < 0.05). The insulin secretion of experimental group was 
      significantly higher than that of normal control and blank control groups (P < 
      0.05), but it was 1/40 and 1147 of natural islet cells (P < 0.05). CONCLUSION: 
      After pancreatic injury, injured pancreas would secrete transcription proteins 
      related to development, differentiation, and repair of pancreas, which can 
      promote the differentiation of BMSCs into IPCs.
FAU - Fei, Yun
AU  - Fei Y
FAU - Xie, Hongbin
AU  - Xie H
FAU - Wang, Yunshuai
AU  - Wang Y
FAU - Qi, Hui
AU  - Qi H
FAU - Deng, Chunyan
AU  - Deng C
FAU - Ren, Lili
AU  - Ren L
FAU - Li, Furong
AU  - Li F
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Insulin)
RN  - 0 (Tissue Extracts)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Glucose
MH  - Hematopoietic Stem Cells
MH  - Insulin/*biosynthesis
MH  - Islets of Langerhans
MH  - Mesenchymal Stem Cells/*physiology
MH  - Pancreas/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Extracts
EDAT- 2014/07/31 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/07/31 06:00
PHST- 2014/07/31 06:00 [entrez]
PHST- 2014/07/31 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2014 May;28(5):625-32.