PMID- 25074488
OWN - NLM
STAT- MEDLINE
DCOM- 20160209
LR  - 20211203
IS  - 1432-2013 (Electronic)
IS  - 0031-6768 (Linking)
VI  - 467
IP  - 6
DP  - 2015 Jun
TI  - Glucose deprivation promotes activation of mTOR signaling pathway and protein 
      synthesis in rat skeletal muscle cells.
PG  - 1357-66
LID - 10.1007/s00424-014-1583-2 [doi]
AB  - Signaling through mammalian target of rapamycin (mTOR) has been shown to play a 
      central role in the regulation of skeletal muscle growth induced by a wide range 
      of stimuli either mechanical or metabolic, such as growth factors and amino 
      acids. Here, we demonstrate that mTOR and its downstream target, the ribosomal S6 
      kinase (p70(S6K)), are activated in L6 myocytes by a short-term glucose 
      deprivation. Such response is specific of skeletal muscle and is likely 
      responsible for the increased rate of protein synthesis and expression of the 
      muscle-specific proteins during recovery from glucose deprivation. Nitric oxide 
      and phosphatidylinositol-3-kinase (PI3K) are upstream positive regulators of mTOR 
      since their pharmacological inhibition prevents the activation of p70(S6K) in 
      response to glucose deprivation. We therefore propose a model of response to a 
      brief period of glucose deprivation that may occur in skeletal muscle cells 
      during resistance exercise and that may lead to protein accretion when blood flow 
      recovers and all nutrients are again available.
FAU - Miniaci, Maria Concetta
AU  - Miniaci MC
AD  - Department of Pharmacy, University of Napoli "Federico II", via D. Montesano 49, 
      80131, Naples, Italy, maria.miniaci@unina.it.
FAU - Dattolo, Maria Gabriella
AU  - Dattolo MG
FAU - Irace, Carlo
AU  - Irace C
FAU - Capuozzo, Antonella
AU  - Capuozzo A
FAU - Santamaria, Rita
AU  - Santamaria R
FAU - Scotto, Pietro
AU  - Scotto P
LA  - eng
PT  - Journal Article
DEP - 20140731
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Caco-2 Cells
MH  - Glucose/*deficiency/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Muscle Contraction
MH  - Myoblasts, Skeletal/*metabolism/physiology
MH  - Nitric Oxide/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - *Protein Biosynthesis
MH  - Rats
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2014/07/31 06:00
MHDA- 2016/02/10 06:00
CRDT- 2014/07/31 06:00
PHST- 2014/05/29 00:00 [received]
PHST- 2014/07/18 00:00 [accepted]
PHST- 2014/07/10 00:00 [revised]
PHST- 2014/07/31 06:00 [entrez]
PHST- 2014/07/31 06:00 [pubmed]
PHST- 2016/02/10 06:00 [medline]
AID - 10.1007/s00424-014-1583-2 [doi]
PST - ppublish
SO  - Pflugers Arch. 2015 Jun;467(6):1357-66. doi: 10.1007/s00424-014-1583-2. Epub 2014 
      Jul 31.