PMID- 25074577
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20141118
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 93
IP  - 1
DP  - 2015 Jan
TI  - Furin mediates brain-derived neurotrophic factor upregulation in cultured rat 
      astrocytes exposed to oxygen-glucose deprivation.
PG  - 189-94
LID - 10.1002/jnr.23455 [doi]
AB  - This study investigated the changes in brain-derived neurotrophic factor (BDNF) 
      expression and the role of furin in BDNF maturation in reactive astrocytes from 
      rats exposed to oxygen-glucose deprivation (OGD). Furin, a proprotein convertase, 
      is upregulated and cleaves certain substrates during hypoxia in cancer cells. In 
      addition, during hypoxia in the central nervous system, astrocytes become 
      reactive and release BDNF to protect neurons. Maturation of BDNF in astrocytes 
      requires furin-mediated endoproteolytic processing of the precursor protein 
      pro-BDNF to BDNF. To expand our knowledge about the role of furin in BDNF 
      maturation in astrocytes, these cells were exposed to OGD, and expression of 
      furin and BDNF was detected by Western blot analysis. Changes in BDNF expression 
      were observed when furin activity was inhibited by furin prosegment. We found 
      that protein expression of BDNF and furin was upregulated, and this upregulation 
      correlated with OGD stimulation. Furin inhibition reduced BDNF maturation and 
      secretion. These results indicate that furin mediates the upregulation of BDNF in 
      reactive astrocytes exposed to OGD and that furin may impact the biological 
      effect of reactive astrocytes.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Chen, Yan
AU  - Chen Y
AD  - Department of Neurology, Zhongnan Hospital, Wuhan University, Wuhan, China.
FAU - Zhang, Junjian
AU  - Zhang J
FAU - Deng, Min
AU  - Deng M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140730
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - EC 3.4.21.75 (Furin)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Furin/*metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glucose/*deficiency
MH  - Oxygen/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Transfection
MH  - Up-Regulation/*physiology
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - furin
OT  - oxygen-glucose deprivation
OT  - reactivated astrocyte
EDAT- 2014/07/31 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/07/31 06:00
PHST- 2014/05/09 00:00 [received]
PHST- 2014/06/18 00:00 [revised]
PHST- 2014/06/27 00:00 [accepted]
PHST- 2014/07/31 06:00 [entrez]
PHST- 2014/07/31 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1002/jnr.23455 [doi]
PST - ppublish
SO  - J Neurosci Res. 2015 Jan;93(1):189-94. doi: 10.1002/jnr.23455. Epub 2014 Jul 30.