PMID- 25075032
OWN - NLM
STAT- MEDLINE
DCOM- 20141111
LR  - 20140730
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 34
IP  - 8
DP  - 2014 Aug
TI  - Evaluating the cytotoxic effects of novel quinone compounds.
PG  - 4077-86
AB  - BACKGROUND/AIM: Quinone-containing compounds can induce cell death in cancer 
      cells and are, therefore, promising lead compounds for the development of novel 
      anti-cancer drugs. MATERIALS AND METHODS: In the present study, we evaluated the 
      cytotoxic effects of fifteen novel synthetic quinone-containing compounds in cell 
      cultures in an attempt to establish structure/activity relationships for these 
      compounds. The compounds were clustered into four groups (1, 2, 3, 4) based on 
      common structural features. In vitro cell cultures were treated for 24 h with the 
      compounds, after which cell viability was assessed by flow cytometry. The 
      APOPercentage assay, the Terminal deoxynucleotidyl transferase mediated dUTP 
      Nick End Labeling (TUNEL) assay and the caspase-3 assay was used to investigate 
      the activation of apoptosis in the cells. RESULTS: Compounds from groups 2 and 4 
      were highly toxic to the cells. The compounds induced apoptosis in some human 
      cancer cell cultures and exhibited low toxicity towards the non-cancerous cell 
      line, KMST-6. The induction of apoptosis in CHO cells was associated with the 
      activation of caspase-3 cleavage, DNA fragmentation and the reactive oxygen 
      species (ROS) generation. CONCLUSION: The present study demonstrates that five of 
      the quinone-containing compounds induced apoptosis in human cancer cells and are 
      therefore promising lead compounds for the development of novel anticancer drugs.
CI  - Copyright(c) 2014 International Institute of Anticancer Research (Dr. John G. 
      Delinassios), All rights reserved.
FAU - Saibu, Morounke
AU  - Saibu M
AD  - Department of Biotechnology, University of the Western Cape, Bellville, South 
      Africa.
FAU - Sagar, Sunil
AU  - Sagar S
AD  - Department of Chemistry, University of the Western Cape, Bellville, South Africa.
FAU - Green, Ivan
AU  - Green I
AD  - Department of Chemistry, University of the Western Cape, Bellville, South Africa.
FAU - Ameer, Farouk
AU  - Ameer F
AD  - Department of Chemistry, University of the Western Cape, Bellville, South Africa.
FAU - Meyer, Mervin
AU  - Meyer M
AD  - Department of Biotechnology, University of the Western Cape, Bellville, South 
      Africa memeyer@uwc.ac.za.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Quinones)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - CHO Cells
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cricetulus
MH  - DNA Fragmentation
MH  - Humans
MH  - Quinones/*pharmacology
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Cancer
OT  - apoptosis
OT  - quinone
OT  - structure/activity relationships
EDAT- 2014/07/31 06:00
MHDA- 2014/11/12 06:00
CRDT- 2014/07/31 06:00
PHST- 2014/07/31 06:00 [entrez]
PHST- 2014/07/31 06:00 [pubmed]
PHST- 2014/11/12 06:00 [medline]
AID - 34/8/4077 [pii]
PST - ppublish
SO  - Anticancer Res. 2014 Aug;34(8):4077-86.