PMID- 25079422
OWN - NLM
STAT- MEDLINE
DCOM- 20141007
LR  - 20151119
IS  - 1001-0742 (Print)
IS  - 1001-0742 (Linking)
VI  - 26
IP  - 4
DP  - 2014 Apr 1
TI  - Integrated biomarkers in wild crucian carp for early warning of water quality in 
      Hun River, North China.
PG  - 909-16
LID - S1001-0742(13)60484-2 [pii]
LID - 10.1016/S1001-0742(13)60484-2 [doi]
AB  - Metabolizing enzymes play important roles in the detoxification of various 
      pollutants in aquatic organisms, thereby they can also be used to provide 
      early-warning signals of environmental risks. Real-time quantitative 
      reverse-transcription polymerase chain reaction assays were developed to quantify 
      cytochrome P450 1A (CYP1A), superoxide dismutase (SOD), glutathione peroxidase 
      (GPx), catalase (CAT), and glutathione-S-transferase (GST) in crucian carp 
      (Carassius auratus). The methods were then used to detect the respective mRNA 
      expression levels in liver tissue in wild crucian carp from the Hun River, North 
      China. CYP1A mRNA expression was significantly up-regulated in fish from stations 
      S5, S6, and S8 (p < 0.05). SOD mRNA expression was significantly down-regulated 
      in downstream areas relative to fish from upstream sites (p < 0.05); GPx and CAT 
      mRNA expression levels were also down-regulated at S9 (p < 0.05). In contrast, 
      GST mRNA expression showed no obvious change between fish collected from up- or 
      downstream areas of the river. Finally, an integrated biomarker response was used 
      to evaluate the integrated impact of pollutants in the Hun River and allow better 
      comprehension of the real toxicological risk of these investigated sites.
CI  - Copyright (c) 2014 The Research Centre for Eco-Environmental Sciences, Chinese 
      Academy of Sciences. Published by Elsevier B.V. All rights reserved.
FAU - Zheng, Binghui
AU  - Zheng B
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment & State 
      Environmental Protection Key Laboratory of Estuarine and Coastal Research, 
      Chinese Research Academy of Environment Sciences, Beijing 100012. Electronic 
      address: zhengbh@craes.org.cn.
FAU - Lei, Kun
AU  - Lei K
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment & State 
      Environmental Protection Key Laboratory of Estuarine and Coastal Research, 
      Chinese Research Academy of Environment Sciences, Beijing 100012.
FAU - Liu, Ruizhi
AU  - Liu R
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment & State 
      Environmental Protection Key Laboratory of Estuarine and Coastal Research, 
      Chinese Research Academy of Environment Sciences, Beijing 100012.
FAU - Song, Shuangshuang
AU  - Song S
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment & State 
      Environmental Protection Key Laboratory of Estuarine and Coastal Research, 
      Chinese Research Academy of Environment Sciences, Beijing 100012.
FAU - An, Lihui
AU  - An L
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment & State 
      Environmental Protection Key Laboratory of Estuarine and Coastal Research, 
      Chinese Research Academy of Environment Sciences, Beijing 100012. Electronic 
      address: anlhui@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Environ Sci (China)
JT  - Journal of environmental sciences (China)
JID - 100967627
RN  - 0 (Biomarkers)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/metabolism
MH  - Biomarkers/*metabolism
MH  - Catalase/metabolism
MH  - *Environmental Monitoring
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Transferase/metabolism
MH  - Goldfish/*metabolism
MH  - Molecular Sequence Data
MH  - Superoxide Dismutase/metabolism
MH  - *Water Quality
OTO - NOTNLM
OT  - Carassius auratus
OT  - Hun River
OT  - integrated biomarker response
OT  - real-time quantitative reverse-transcription polymerase chain reaction
OT  - water quality
EDAT- 2014/08/01 06:00
MHDA- 2014/10/08 06:00
CRDT- 2014/08/01 06:00
PHST- 2013/05/20 00:00 [received]
PHST- 2013/09/10 00:00 [revised]
PHST- 2013/10/23 00:00 [accepted]
PHST- 2014/08/01 06:00 [entrez]
PHST- 2014/08/01 06:00 [pubmed]
PHST- 2014/10/08 06:00 [medline]
AID - S1001-0742(13)60484-2 [pii]
AID - 10.1016/S1001-0742(13)60484-2 [doi]
PST - ppublish
SO  - J Environ Sci (China). 2014 Apr 1;26(4):909-16. doi: 
      10.1016/S1001-0742(13)60484-2.