PMID- 25080025
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 406
IP  - 25
DP  - 2014 Oct
TI  - Evaluation of molecularly imprinted polymers using 2',3',5'-tri-O-acyluridines as 
      templates for pyrimidine nucleoside recognition.
PG  - 6275-84
LID - 10.1007/s00216-014-8017-z [doi]
AB  - In this paper, we describe the synthesis and evaluation of molecularly imprinted 
      polymers (MIPs), prepared using 2',3',5'-tri-O-acyluridines as 'dummy' templates, 
      for the selective recognition of uridine nucleosides. The MIPs were synthesised 
      using a non-covalent approach with 2,6-bis-acrylamidopyridine (BAAPy) acting as 
      the binding monomer and ethylene glycol dimethacrylate (EGDMA) as the 
      cross-linking agent. The MIPs were evaluated in terms of capacity, selectivity 
      and specificity by analytical and frontal liquid chromatography measurements. The 
      results obtained in organic mobile phases suggest that the nucleosides are 
      specifically bound to the polymer by the complementary hydrogen bonding motifs of 
      the binding monomer and the nucleoside bases. The MIPs exhibited relatively high 
      imprinting factors for 2',3',5'-tri-O-acyluridines, while they did not show any 
      binding capacity for other nucleosides lacking the imide moiety on their base. 
      Moreover, the presence of ester-COO groups in the EGDMA cross-linker may lead to 
      the formation of additional hydrogen bonds with the 2',3' and/or 5'-OH of sugar 
      part, allowing enhancement of the recognition of the uridine nucleosides. In 
      aqueous media, results show that the binding is driven by hydrophobic 
      interactions.
FAU - Krstulja, Aleksandra
AU  - Krstulja A
AD  - ICOA UMR CNRS 7311, Universite d'Orleans, 45067, Orleans Cedex 2, France.
FAU - Lettieri, Stefania
AU  - Lettieri S
FAU - Hall, Andrew J
AU  - Hall AJ
FAU - Delepee, Raphael
AU  - Delepee R
FAU - Favetta, Patrick
AU  - Favetta P
FAU - Agrofoglio, Luigi A
AU  - Agrofoglio LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140731
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Polymers)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - Hydrogen Bonding
MH  - Molecular Imprinting
MH  - Polymers/chemical synthesis/*chemistry
MH  - Stereoisomerism
MH  - Uridine/*chemistry
EDAT- 2014/08/01 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/08/01 06:00
PHST- 2014/04/22 00:00 [received]
PHST- 2014/07/01 00:00 [accepted]
PHST- 2014/06/02 00:00 [revised]
PHST- 2014/08/01 06:00 [entrez]
PHST- 2014/08/01 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1007/s00216-014-8017-z [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2014 Oct;406(25):6275-84. doi: 10.1007/s00216-014-8017-z. Epub 
      2014 Jul 31.