PMID- 25082526
OWN - NLM
STAT- MEDLINE
DCOM- 20141009
LR  - 20211021
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1359
DP  - 2014 Sep 12
TI  - Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry 
      for quantitative parallel reaction monitoring of peptide abundance and 
      single-shot proteomic analysis of a human cell line.
PG  - 303-8
LID - S0021-9673(14)01093-0 [pii]
LID - 10.1016/j.chroma.2014.07.024 [doi]
AB  - We coupled capillary zone electrophoresis (CZE) with an ultrasensitive 
      electrokinetically pumped nanospray ionization source for tandem mass 
      spectrometry (MS/MS) analysis of complex proteomes. We first used the system for 
      the parallel reaction monitoring (PRM) analysis of angiotensin II spiked in 
      0.45mg/mL of bovine serum albumin (BSA) digest. A calibration curve was generated 
      between the loading amount of angiotensin II and intensity of angiotensin II 
      fragment ions. CZE-PRM generated a linear calibration curve across over 4.5 
      orders of magnitude dynamic range corresponding to angiotensin II loading amount 
      from 2amole to 150fmole. The relative standard deviations (RSDs) of migration 
      time were <4% and the RSDs of fragment ion intensity were  approximately 20% or less except 
      150fmole angiotensin II loading amount data ( approximately 36% RSD). We further applied the 
      system for the first bottom up proteomic analysis of a human cell line using 
      CZE-MS/MS. We generated 283 protein identifications from a 1h long, single-shot 
      CZE MS/MS analysis of the MCF7 breast cancer cell line digest, corresponding to 
       approximately 80ng loading amount. The MCF7 digest was fractionated using a C18 solid phase 
      extraction column; single-shot analysis of a single fraction resulted in 468 
      protein identifications, which is by far the largest number of protein 
      identifications reported for a mammalian proteomic sample using CZE.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Sun, Liangliang
AU  - Sun L
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA.
FAU - Zhu, Guijie
AU  - Zhu G
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA.
FAU - Mou, Si
AU  - Mou S
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA.
FAU - Zhao, Yimeng
AU  - Zhao Y
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA.
FAU - Champion, Matthew M
AU  - Champion MM
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA.
FAU - Dovichi, Norman J
AU  - Dovichi NJ
AD  - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, 
      IN 46556, USA. Electronic address: ndovichi@nd.edu.
LA  - eng
GR  - R01 GM096767/GM/NIGMS NIH HHS/United States
GR  - R01GM096767/GM/NIGMS NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140717
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Peptides)
RN  - 0 (Proteins)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Peptides/*chemistry/isolation & purification
MH  - Proteins/*chemistry
MH  - Proteomics/*methods
MH  - Serum Albumin, Bovine/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
PMC - PMC4141985
MID - NIHMS618163
OTO - NOTNLM
OT  - CZE-ESI-MS/MS
OT  - CZE-PRM
OT  - Human cell lysate digest
OT  - Shotgun proteomics
OT  - Ultrasensitive electrokinetically pumped sheath flow nanospray interface
EDAT- 2014/08/02 06:00
MHDA- 2014/10/10 06:00
PMCR- 2015/09/12
CRDT- 2014/08/02 06:00
PHST- 2014/04/09 00:00 [received]
PHST- 2014/06/25 00:00 [revised]
PHST- 2014/07/10 00:00 [accepted]
PHST- 2014/08/02 06:00 [entrez]
PHST- 2014/08/02 06:00 [pubmed]
PHST- 2014/10/10 06:00 [medline]
PHST- 2015/09/12 00:00 [pmc-release]
AID - S0021-9673(14)01093-0 [pii]
AID - 10.1016/j.chroma.2014.07.024 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Sep 12;1359:303-8. doi: 10.1016/j.chroma.2014.07.024. Epub 
      2014 Jul 17.