PMID- 25082660
OWN - NLM
STAT- MEDLINE
DCOM- 20150914
LR  - 20220410
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 112
IP  - 2
DP  - 2015 Feb
TI  - Effect of liquid hot water pretreatment severity on properties of hardwood lignin 
      and enzymatic hydrolysis of cellulose.
PG  - 252-62
LID - 10.1002/bit.25349 [doi]
AB  - Lignin, one of the major components of lignocellulosic biomass, plays an 
      inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the 
      role of lignin in pretreated hardwood, where extents of cellulose hydrolysis 
      decrease, rather than increase with increasing severity of liquid hot water 
      pretreatment. Hardwood pretreated with liquid hot water at severities ranging 
      from log Ro  = 8.25 to 12.51 resulted in 80-90% recovery of the initial lignin in 
      the residual solids. The ratio of acid insoluble lignin (AIL) to acid soluble 
      lignin (ASL) increased and the formation of spherical lignin droplets on the cell 
      wall surface was observed as previously reported in the literature. When lignins 
      were isolated from hardwoods pretreated at increasing severities and 
      characterized based on glass transition temperature (Tg ), the Tg of isolated 
      lignins was found to increase from 171 to 180 degrees C as the severity increased from 
      log Ro  = 10.44 to 12.51. The increase in Tg suggested that the condensation 
      reactions of lignin molecules occurred during pretreatment and altered the lignin 
      structure. The contribution of the changes in lignin properties to enzymatic 
      hydrolysis were examined by carrying out Avicel hydrolysis in the presence of 
      isolated lignins. Lignins derived from more severely pretreated hardwoods had 
      higher Tg values and showed more pronounced inhibition of enzymatic hydrolysis.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Ko, Ja Kyong
AU  - Ko JK
AD  - Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, 
      Indiana, 47907-2022; Department of Agricultural and Biological Engineering, 
      Purdue University, West Lafayette, Indiana, 47907-2022.
FAU - Kim, Youngmi
AU  - Kim Y
FAU - Ximenes, Eduardo
AU  - Ximenes E
FAU - Ladisch, Michael R
AU  - Ladisch MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
DEP - 20141010
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 059QF0KO0R (Water)
RN  - 9004-34-6 (Cellulose)
RN  - 9005-53-2 (Lignin)
SB  - IM
MH  - Biomass
MH  - Cellulose/*chemistry
MH  - Glass
MH  - *Hot Temperature
MH  - Hydrolysis
MH  - Lignin/*chemistry
MH  - Water/*chemistry
OTO - NOTNLM
OT  - AIL/ASL ratio
OT  - hardwood
OT  - lignin
OT  - lignin glass transition temperature
OT  - liquid hot water pretreatment
OT  - severity
EDAT- 2014/08/02 06:00
MHDA- 2015/09/15 06:00
CRDT- 2014/08/02 06:00
PHST- 2014/04/24 00:00 [received]
PHST- 2014/07/02 00:00 [revised]
PHST- 2014/07/21 00:00 [accepted]
PHST- 2014/08/02 06:00 [entrez]
PHST- 2014/08/02 06:00 [pubmed]
PHST- 2015/09/15 06:00 [medline]
AID - 10.1002/bit.25349 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2015 Feb;112(2):252-62. doi: 10.1002/bit.25349. Epub 2014 Oct 
      10.