PMID- 25083284
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140801
LR  - 20211021
IS  - 2050-6406 (Print)
IS  - 2050-6414 (Electronic)
IS  - 2050-6406 (Linking)
VI  - 2
IP  - 4
DP  - 2014 Aug
TI  - Tetramer-visualized gluten-specific CD4+ T cells in blood as a potential 
      diagnostic marker for coeliac disease without oral gluten challenge.
PG  - 268-78
LID - 10.1177/2050640614540154 [doi]
AB  - BACKGROUND: Diagnosing coeliac disease (CD) can be challenging, despite highly 
      specific autoantibodies and typical mucosal changes in the small intestine. The 
      T-cell response to gluten is a hallmark of the disease that has been hitherto 
      unexploited in clinical work-up. OBJECTIVES: We aimed to develop a new method 
      that directly visualizes and characterizes gluten-reactive CD4+ T cells in blood, 
      independently of gluten challenge, and to explore its diagnostic potential. 
      METHODS: We performed bead-enrichment of DQ2.5-glia-alpha1a and DQ2.5-glia-alpha2 
      tetramer+ cells in the blood of control individuals, treated (TCD) and untreated 
      patients (UCD). We visualized these cells by flow cytometry, sorted them and 
      cloned them. We assessed their specificity by antigen stimulation and re-staining 
      with tetramers. RESULTS: We detected significantly more gliadin-tetramer+ CD4+ 
      effector memory T cells (TEM) in UCD and TCD patients, compared to controls. 
      Significantly more gliadin-tetramer+ TEM in the CD patients than in controls 
      expressed the gut-homing marker integrin-beta7. CONCLUSION: Quantification of 
      gut-homing, gluten-specific TEM in peripheral blood, visualized with human 
      leukocyte antigen (HLA) -tetramers, may be used to distinguish CD patients from 
      healthy individuals. Easy access to gluten-reactive blood T cells from diseased 
      and healthy individuals may lead to new insights on the disease-driving CD4+ T 
      cells in CD.
FAU - Christophersen, Asbjorn
AU  - Christophersen A
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway.
FAU - Raki, Melinda
AU  - Raki M
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway.
FAU - Bergseng, Elin
AU  - Bergseng E
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway.
FAU - Lundin, Knut Ea
AU  - Lundin KE
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway ; Department of 
      Transplantation Medicine, Division of Cancer Medicine, Surgery and 
      Transplantation, Oslo University Hospital - Rikshospitalet, Oslo, Norway.
FAU - Jahnsen, Jorgen
AU  - Jahnsen J
AD  - Department of Gastroenterology, Oslo University Hospital - Ulleval, Oslo, Norway.
FAU - Sollid, Ludvig M
AU  - Sollid LM
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway.
FAU - Qiao, Shuo-Wang
AU  - Qiao SW
AD  - Centre for Immune Regulation and Department of Immunology, University of Oslo and 
      Oslo University Hospital - Rikshospitalet, Oslo, Norway.
LA  - eng
PT  - Journal Article
PL  - England
TA  - United European Gastroenterol J
JT  - United European gastroenterology journal
JID - 101606807
EIN - United European Gastroenterol J. 2014 Dec;2(6):550. PMID: 25761984
PMC - PMC4114117
OTO - NOTNLM
OT  - Blood test
OT  - T cells
OT  - celiac disease
OT  - diagnostic marker
OT  - diagnostics
OT  - gliadin
OT  - gluten
OT  - gluten reactivity
OT  - histology
OT  - human leukocyte antigen
OT  - immunology
EDAT- 2014/08/02 06:00
MHDA- 2014/08/02 06:01
PMCR- 2014/08/01
CRDT- 2014/08/02 06:00
PHST- 2014/02/19 00:00 [received]
PHST- 2014/04/22 00:00 [revised]
PHST- 2014/05/22 00:00 [accepted]
PHST- 2014/08/02 06:00 [entrez]
PHST- 2014/08/02 06:00 [pubmed]
PHST- 2014/08/02 06:01 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1177_2050640614540154 [pii]
AID - 10.1177/2050640614540154 [doi]
PST - ppublish
SO  - United European Gastroenterol J. 2014 Aug;2(4):268-78. doi: 
      10.1177/2050640614540154.