PMID- 25084402
OWN - NLM
STAT- MEDLINE
DCOM- 20160726
LR  - 20181202
IS  - 1001-0742 (Print)
IS  - 1001-0742 (Linking)
VI  - 21 Suppl 1
DP  - 2009
TI  - Highly sensitive flow-biosensor for toxic phenolic compounds using tyrosinase and 
      acridine orange-adsorbed carbon felt.
PG  - S100-4
LID - 10.1016/S1001-0742(09)60048-6 [doi]
AB  - Tyrosinase (TYR, EC 1.14.18.1) was physically adsorbed onto a carbon felt (CF) 
      together with acridine orange (AO). Coadsorption of AO was essential to prevent 
      the denaturation of the TYR at the CF surface. The resulting TYR and 
      AO-coadsorbed CF (TYR/AO-CF) was successfully utilized as a detection unit of 
      novel and highly sensitive amperometric flow-biosensor for toxic chlorophenol 
      compounds. Standard solutions of phenolic compounds (200 muL) were injected, and 
      the cathodic peak currents due to the reduction current of o-quinones produced by 
      the TYR-catalyzed oxidation (phenolase activity) were detected at the applied 
      potential of -50 mV vs. Ag/AgCl. In this reaction, the electrochemically 
      generated catechol compounds from o-quinones are re-oxidized repeatedly by 
      catecholase activity of the TYR, leading to a sufficient amplified signal. The 
      TYR/AO-CF exhibited much higher selectivity toward p-chlorophenol as compared 
      with other chlorophenol compounds. When 0.1 mol/L phosphate buffer (pH 7.0) was 
      used as a carrier at flow rate of 3.0 mL/min, cathodic peaks for p-chlorophenol 
      was linear in the concentration range between 0.1 and 10 mumol/L (sensitivity: 
      1.41(mA.L)/mmol) with sampling rate (30 samples/h), and the detection limit of 
      p-chlorophenol was found to be 2.13 x 10(8) mol/L (S/N = 3. The ratio of signal 
      and noise is 3). The TYR/AO-CF kept more than 80% of original activity after the 
      storage in 0.1 mol/L phosphate buffer (pH 7.0) containing 0.2 mmol/L AO at 4 degrees C.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Material Science and Engineering, Saitama Institute of Technology, 
      Fukaya 369-0293, Japan; School of Chemical Engineering, University of Science and 
      Technology Liaoning, Anshan 114501, China.
FAU - Hasebe, Yasushi
AU  - Hasebe Y
AD  - Department of Material Science and Engineering, Saitama Institute of Technology, 
      Fukaya 369-0293, Japan; Department of Life Science and Green Chemistry, Saitama 
      Institute of Technology, Fukaya 369-0293, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Environ Sci (China)
JT  - Journal of environmental sciences (China)
JID - 100967627
RN  - 0 (Carbon Fiber)
RN  - 0 (Chlorophenols)
RN  - 0 (Phenols)
RN  - 0 (Solutions)
RN  - 3DLC36A01X (4-chlorophenol)
RN  - 7440-44-0 (Carbon)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Adsorption
MH  - Biocatalysis
MH  - Biosensing Techniques/*methods
MH  - Carbon/*chemistry
MH  - Carbon Fiber
MH  - Chlorophenols/chemistry
MH  - Electrochemistry
MH  - Flow Injection Analysis
MH  - Hydroxylation
MH  - Microscopy, Electron, Scanning
MH  - Monophenol Monooxygenase/*metabolism
MH  - Phenols/*analysis/*toxicity
MH  - Solutions
MH  - Substrate Specificity
EDAT- 2009/01/01 00:00
MHDA- 2016/07/28 06:00
CRDT- 2014/08/02 06:00
PHST- 2014/08/02 06:00 [entrez]
PHST- 2009/01/01 00:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - S1001-0742(09)60048-6 [pii]
AID - 10.1016/S1001-0742(09)60048-6 [doi]
PST - ppublish
SO  - J Environ Sci (China). 2009;21 Suppl 1:S100-4. doi: 
      10.1016/S1001-0742(09)60048-6.