PMID- 25085431
OWN - NLM
STAT- MEDLINE
DCOM- 20150519
LR  - 20211021
IS  - 1347-4715 (Electronic)
IS  - 1342-078X (Print)
IS  - 1342-078X (Linking)
VI  - 19
IP  - 5
DP  - 2014 Sep
TI  - Voluntary exercise under a food restriction condition decreases blood 
      branched-chain amino acid levels, in addition to improvement of glucose and lipid 
      metabolism, in db mice, animal model of type 2 diabetes.
PG  - 339-47
LID - 10.1007/s12199-014-0400-z [doi]
AB  - OBJECTIVES: Exercise is effective for preventing the onset and development of 
      type 2 diabetes mellitus (T2DM) in human cases; however, the effect of exercise 
      on the pathophysiology using animal models of T2DM has not been fully evaluated. 
      METHODS: We applied voluntary exercise under pair-fed (P) conditions in db mice, 
      an animal model of T2DM. Exercising (Ex) and sedentary (Se) mice were placed in a 
      cage, equipped with a free or locked running wheel, for 4 weeks, respectively. 
      The amount of food consumed by ad libitum-fed wild-type mice under the Se 
      condition (ad-WT) was supplied to all mice, except ad libitum db mice (ad-db). 
      Blood parameters and expression of the genes involved in nutrient metabolism were 
      analyzed. RESULTS: PEx-db (pair-fed and exercising) mice showed significantly 
      lower HbA1c, body weight and liver weight than PSe-db and ad-db mice. Decreased 
      hepatic triglycerides in PEx-db mice corresponded to a lower expression of 
      lipogenic enzyme genes in the liver. Moreover, PEx-db mice showed significantly 
      lower plasma branched-chain amino acids (BCAA), arginine, proline, and tyrosine, 
      in addition to increased skeletal muscle (SM) weight, than PSe-db and ad-db mice, 
      in spite of little influence on the expression of the BCAA transaminase gene, in 
      SM and WAT. CONCLUSION: We found that exercise under a food restriction condition 
      decreases several amino acids, including BCAA, and may improve insulin 
      sensitivity more than mere food restriction. We propose that the decreased 
      concentration of blood amino acids may be a valuable marker evaluating the 
      effects of exercise on diabetic conditions.
FAU - Marchianti, Ancah Caesarina Novi
AU  - Marchianti AC
AD  - Department of Hygiene and Health Promotion Medicine, Kagoshima University 
      Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, 
      890-8544, Japan.
FAU - Arimura, Emi
AU  - Arimura E
FAU - Ushikai, Miharu
AU  - Ushikai M
FAU - Horiuchi, Masahisa
AU  - Horiuchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140802
PL  - Japan
TA  - Environ Health Prev Med
JT  - Environmental health and preventive medicine
JID - 9609642
RN  - 0 (Amino Acids, Branched-Chain)
RN  - 0 (RNA, Messenger)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipose Tissue, White/chemistry/metabolism
MH  - Amino Acids, Branched-Chain/metabolism
MH  - Animals
MH  - *Caloric Restriction
MH  - Diabetes Mellitus, Type 2/diet therapy/metabolism/*therapy
MH  - Disease Models, Animal
MH  - Exercise
MH  - Gene Expression
MH  - Glucose/metabolism
MH  - Humans
MH  - Insulin Resistance
MH  - Lipid Metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/metabolism
MH  - *Physical Conditioning, Animal
MH  - RNA, Messenger/genetics/metabolism
PMC - PMC4166586
EDAT- 2014/08/03 06:00
MHDA- 2015/05/20 06:00
PMCR- 2015/09/01
CRDT- 2014/08/03 06:00
PHST- 2014/05/14 00:00 [received]
PHST- 2014/07/13 00:00 [accepted]
PHST- 2014/08/03 06:00 [entrez]
PHST- 2014/08/03 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - 400 [pii]
AID - 10.1007/s12199-014-0400-z [doi]
PST - ppublish
SO  - Environ Health Prev Med. 2014 Sep;19(5):339-47. doi: 10.1007/s12199-014-0400-z. 
      Epub 2014 Aug 2.