PMID- 25086066
OWN - NLM
STAT- MEDLINE
DCOM- 20151113
LR  - 20211021
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 127
IP  - Pt 20
DP  - 2014 Oct 15
TI  - Dictyostelium LvsB has a regulatory role in endosomal vesicle fusion.
PG  - 4356-67
LID - 10.1242/jcs.138123 [doi]
AB  - Defects in human lysosomal-trafficking regulator (Lyst) are associated with the 
      lysosomal disorder Chediak-Higashi syndrome. The absence of Lyst results in the 
      formation of enlarged lysosome-related compartments, but the mechanism for how 
      these compartments arise is not well established. Two opposing models have been 
      proposed to explain Lyst function. The fission model describes Lyst as a positive 
      regulator of fission from lysosomal compartments, whereas the fusion model 
      identifies Lyst as a negative regulator of fusion between lysosomal vesicles. 
      Here, we used assays that can distinguish between defects in vesicle fusion 
      versus fission. We compared the phenotype of Dictyostelium discoideum cells 
      defective in LvsB, the ortholog of Lyst, with that of two known fission defect 
      mutants (mu3- and WASH-null mutants). We found that the temporal localization 
      characteristics of the post-lysosomal marker vacuolin, as well as vesicular 
      acidity and the fusion dynamics of LvsB-null cells are distinct from those of 
      both mu3- and WASH-null fission defect mutants. These distinctions are predicted 
      by the fusion defect model and implicate LvsB as a negative regulator of vesicle 
      fusion.
CI  - (c) 2014. Published by The Company of Biologists Ltd.
FAU - Falkenstein, Kristin
AU  - Falkenstein K
AD  - Section of Molecular Cell and Developmental Biology and Institute for Cellular 
      and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
FAU - De Lozanne, Arturo
AU  - De Lozanne A
AD  - Section of Molecular Cell and Developmental Biology and Institute for Cellular 
      and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA 
      a.delozanne@utexas.edu.
LA  - eng
GR  - R01 GM048745/GM/NIGMS NIH HHS/United States
GR  - R21 AI078136/AI/NIAID NIH HHS/United States
GR  - GM48745/GM/NIGMS NIH HHS/United States
GR  - AI078136/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140801
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (LYST protein, human)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (vac A protein, Dictyostelium discoideum)
SB  - IM
MH  - Biological Evolution
MH  - Chediak-Higashi Syndrome/*genetics
MH  - Cytoskeletal Proteins/metabolism
MH  - Dictyostelium
MH  - Endosomes/metabolism
MH  - Humans
MH  - Lysosomes/genetics/*metabolism
MH  - Membrane Fusion/genetics
MH  - Phagocytosis/genetics
MH  - Phenotype
MH  - Protozoan Proteins/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Vesicular Transport Proteins/genetics/*metabolism
PMC - PMC4197084
OTO - NOTNLM
OT  - Chediak-Higashi syndrome
OT  - Endosome fusion
OT  - LvsB
OT  - Lyst
EDAT- 2014/08/03 06:00
MHDA- 2015/11/14 06:00
PMCR- 2015/10/15
CRDT- 2014/08/03 06:00
PHST- 2014/08/03 06:00 [entrez]
PHST- 2014/08/03 06:00 [pubmed]
PHST- 2015/11/14 06:00 [medline]
PHST- 2015/10/15 00:00 [pmc-release]
AID - jcs.138123 [pii]
AID - 10.1242/jcs.138123 [doi]
PST - ppublish
SO  - J Cell Sci. 2014 Oct 15;127(Pt 20):4356-67. doi: 10.1242/jcs.138123. Epub 2014 
      Aug 1.