PMID- 25086213 OWN - NLM STAT- MEDLINE DCOM- 20150615 LR - 20191210 IS - 1872-9096 (Electronic) IS - 0166-3542 (Linking) VI - 110 DP - 2014 Oct TI - Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro. PG - 60-9 LID - S0166-3542(14)00210-1 [pii] LID - 10.1016/j.antiviral.2014.07.011 [doi] AB - Virus replication depends upon host-cell processes in infected cells, and this is true for porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of PRRS that is a worldwide threat to the swine industry. Heme oxygenase-1 (HO-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. Our previous research suggested that HO-1 may play an important role in PRRSV infection. However, the function of HO-1 in PRRSV infection is unclear. In the present study, Marc-145, PK-15(CD163) cell lines and porcine alveolar macrophages (PAMs) were used to evaluate the effects of HO-1 induction and over-expression on the replication of two different PRRSV strains. Induction of HO-1 markedly decreased the replication of PRRSV strains in the different cells. Similarly, adenoviral-mediated over-expression of HO-1 also greatly decreased the replication of PRRSV. In contrast, ablation of HO-1 using small interfering RNA concomitantly increased PRRSV replication. Therefore, the data were consistent with HO-1 acting as an antiviral factor and these findings suggested that over-expression or induction of HO-1 may provide a potential therapeutic strategy against PRRSV infection. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Xiao, Shuqi AU - Xiao S AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Zhang, Angke AU - Zhang A AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Zhang, Chong AU - Zhang C AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Ni, Huaibo AU - Ni H AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Gao, Jiming AU - Gao J AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Wang, Chengbao AU - Wang C AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Zhao, Qin AU - Zhao Q AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Wang, Xiangpeng AU - Wang X AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Wang, Xue AU - Wang X AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Ma, Chao AU - Ma C AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Liu, Hongliang AU - Liu H AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Li, Na AU - Li N AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Mu, Yang AU - Mu Y AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Sun, Yani AU - Sun Y AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. FAU - Zhang, Gaiping AU - Zhang G AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China. FAU - Hiscox, Julian A AU - Hiscox JA AD - Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK. FAU - Hsu, Walter H AU - Hsu WH AD - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. FAU - Zhou, En-Min AU - Zhou EM AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi 712100, China. Electronic address: zhouem@nwsuaf.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140730 PL - Netherlands TA - Antiviral Res JT - Antiviral research JID - 8109699 RN - 0 (RNA, Small Interfering) RN - EC 1.14.14.18 (Heme Oxygenase-1) SB - IM MH - Animals MH - Cell Line MH - Chlorocebus aethiops MH - Heme Oxygenase-1/biosynthesis/*genetics MH - Immunity, Innate/genetics MH - Macrophages, Alveolar/*enzymology MH - Porcine Reproductive and Respiratory Syndrome/*genetics/therapy MH - Porcine respiratory and reproductive syndrome virus/*growth & development MH - RNA Interference MH - RNA, Small Interfering MH - Swine MH - Virus Replication/*genetics OTO - NOTNLM OT - Antivirus factor OT - HO-1 OT - Highly pathogenic PRRSV OT - PRRSV OT - siRNA EDAT- 2014/08/03 06:00 MHDA- 2015/06/16 06:00 CRDT- 2014/08/03 06:00 PHST- 2014/01/25 00:00 [received] PHST- 2014/07/19 00:00 [revised] PHST- 2014/07/22 00:00 [accepted] PHST- 2014/08/03 06:00 [entrez] PHST- 2014/08/03 06:00 [pubmed] PHST- 2015/06/16 06:00 [medline] AID - S0166-3542(14)00210-1 [pii] AID - 10.1016/j.antiviral.2014.07.011 [doi] PST - ppublish SO - Antiviral Res. 2014 Oct;110:60-9. doi: 10.1016/j.antiviral.2014.07.011. Epub 2014 Jul 30.