PMID- 25088305
OWN - NLM
STAT- MEDLINE
DCOM- 20150413
LR  - 20161125
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 5
IP  - 9
DP  - 2014 Sep
TI  - Esculetin inhibits the inflammatory response by inducing heme oxygenase-1 in 
      cocultured macrophages and adipocytes.
PG  - 2371-7
LID - 10.1039/c4fo00351a [doi]
AB  - Obesity is associated with chronic low-grade inflammation of adipose tissue. In 
      this study, we investigated the anti-inflammatory effects of esculetin (ECT) 
      through up-regulation of heme oxygenase-1 (HO-1) in cocultured macrophages and 
      adipocytes. RAW264.7 macrophages and differentiated 3T3-L1 adipocytes were 
      cocultured in serum-free Dulbecco's modified Eagle's medium with or without ECT 
      for 24 h. Nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and monocyte 
      chemoattractant protein-1 (MCP-1) production was measured in the coculture 
      supernatant. ECT decreased the secretion of NO, TNF-alpha, and MCP-1. The expression 
      of adipogenic proteins, including peroxisome proliferator-activated receptors gamma 
      (PPARgamma) and CCAAT/enhancer binding protein alpha (C/EBPalpha) in cocultured adipocytes 
      and inducible nitric oxide synthase (iNOS) in cocultured macrophages, was 
      inhibited by ECT. Additionally, HO-1 expression was induced in cocultured 
      macrophages and adipocytes. Silencing of HO-1 expression increased the production 
      of NO, TNF-alpha, and MCP-1 in cocultured cells, in spite of the presence of ECT. 
      This study demonstrated that ECT exhibited anti-inflammatory properties by 
      inhibiting the production of proinflammatory cytokines in the interaction between 
      adipocytes and macrophages through HO-1 expression. ECT may have the potential to 
      improve chronic inflammation in obesity.
FAU - Kim, Younghwa
AU  - Kim Y
AD  - Department of Food Science and Technology, College of Agriculture, Life and 
      Environment Sciences, Chungbuk National University, 52 Naesudong-ro, 
      Heungdeok-gu, Cheongju, Chungbuk 361-763, Korea. junsoo@chungbuk.ac.kr.
FAU - Park, Yooheon
AU  - Park Y
FAU - Namkoong, Seulgi
AU  - Namkoong S
FAU - Lee, Junsoo
AU  - Lee J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (PPAR gamma)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Umbelliferones)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - SM2XD6V944 (esculetin)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/immunology
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics/immunology
MH  - Coculture Techniques
MH  - Heme Oxygenase-1/genetics/*immunology
MH  - Macrophages/*drug effects/immunology
MH  - Mice
MH  - PPAR gamma/genetics/immunology
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
MH  - Umbelliferones/*pharmacology
EDAT- 2014/08/05 06:00
MHDA- 2015/04/14 06:00
CRDT- 2014/08/05 06:00
PHST- 2014/08/05 06:00 [entrez]
PHST- 2014/08/05 06:00 [pubmed]
PHST- 2015/04/14 06:00 [medline]
AID - 10.1039/c4fo00351a [doi]
PST - ppublish
SO  - Food Funct. 2014 Sep;5(9):2371-7. doi: 10.1039/c4fo00351a.