PMID- 25088816
OWN - NLM
STAT- MEDLINE
DCOM- 20150605
LR  - 20220408
IS  - 1872-7484 (Electronic)
IS  - 1566-0702 (Linking)
VI  - 185
DP  - 2014 Oct
TI  - Does hepatic vagus nerve modulate the progression of biliary fibrosis in rats?
PG  - 67-75
LID - S1566-0702(14)00099-X [pii]
LID - 10.1016/j.autneu.2014.07.005 [doi]
AB  - Recent studies have shown that vagus nerve activation inhibits cytokine 
      production in a variety of non-neural cells though activation of alpha7 nicotinic 
      acetylcholine receptor (alpha7nAChR). Since chronic inflammation plays a pivotal role 
      in liver fibrosis, this study was designed to investigate the role of hepatic 
      vagus nerve in the progression of hepatic fibrosis in rats. Cirrhosis was induced 
      by chronic ligation of the bile duct. Hepatic hydroxyproline level, portal 
      pressure, serum transaminase level, hepatic TIMP-1 (tissue inhibitor of 
      metalloproteinase-1) and MCP-1 (monocyte chemoattractant peptide-1) expression 
      were measured in order to assess the progression of liver cirrhosis. alpha7nAChR 
      expression was assessed using RT-PCR as well as immunostaining. RT-PCR analysis 
      of the liver showed that alpha7nAChR mRNA is expressed in rat liver. Immunostaining 
      study demonstrated that hepatic alpha7nAChR is mainly expressed in the hepatocytes of 
      cirrhotic liver with minimum alpha7nAChR expression in biliary epithelium or 
      myofibroblasts. Bile duct ligation was associated with portal hypertension, 
      increased hepatic hydroxyproline level as well as TIMP-1 and MCP-1 expression in 
      the liver. However neither selective hepatic vagotomy nor methyllycaconitine (an 
      alpha7nAChR antagonist) could significantly affect development of portal hypertension 
      or hepatic fibrosis in rats. Selective hepatic vagotomy could only attenuate 
      serum aspartate aminotransferase level in bile duct ligated rats but did not have 
      a significant effect on hepatic inflammation as assessed by MCP-1 mRNA 
      expression. Our study provides evidence against a crucial role for the hepatic 
      vagus nerve as an intrinsic protective mechanism in modulation of hepatic 
      fibrosis in a rat model of biliary cirrhosis.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Hajiasgharzadeh, Khalil
AU  - Hajiasgharzadeh K
AD  - Department of Physiology, School of Medical Sciences, Tarbiat Modares University, 
      Tehran, Iran.
FAU - Tavangar, Seyed Mohammad
AU  - Tavangar SM
AD  - Department of Pathology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Javan, Mohammad
AU  - Javan M
AD  - Department of Physiology, School of Medical Sciences, Tarbiat Modares University, 
      Tehran, Iran.
FAU - Dehpour, Ahmad R
AU  - Dehpour AR
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Mani, Ali R
AU  - Mani AR
AD  - Department of Physiology, School of Medical Sciences, Tarbiat Modares University, 
      Tehran, Iran. Electronic address: mani@modares.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140717
PL  - Netherlands
TA  - Auton Neurosci
JT  - Autonomic neuroscience : basic & clinical
JID - 100909359
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Nicotinic Antagonists)
RN  - 0 (RNA, Messenger)
RN  - 0 (TIMP1 protein, rat)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
RN  - 21019-30-7 (methyllycaconitine)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - X8YN71D5WC (Aconitine)
SB  - IM
MH  - Aconitine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Aspartate Aminotransferases/blood
MH  - Chemokine CCL2/metabolism
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Epithelium/drug effects/pathology/physiopathology
MH  - Hepatocytes/drug effects/metabolism/pathology
MH  - Liver/drug effects/pathology/physiopathology
MH  - Liver Cirrhosis, Biliary/drug therapy/pathology/*physiopathology/surgery
MH  - Male
MH  - Myofibroblasts/drug effects/pathology/physiology
MH  - Nicotinic Antagonists/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats, Sprague-Dawley
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Vagotomy
MH  - Vagus Nerve/drug effects/pathology/*physiopathology
MH  - alpha7 Nicotinic Acetylcholine Receptor/antagonists & inhibitors/*metabolism
OTO - NOTNLM
OT  - Cirrhosis
OT  - Fibrosis
OT  - Inflammation
OT  - Vagus nerve
OT  - alpha7 nicotinic acetylcholine receptor
EDAT- 2014/08/05 06:00
MHDA- 2015/06/06 06:00
CRDT- 2014/08/05 06:00
PHST- 2014/04/16 00:00 [received]
PHST- 2014/06/23 00:00 [revised]
PHST- 2014/07/12 00:00 [accepted]
PHST- 2014/08/05 06:00 [entrez]
PHST- 2014/08/05 06:00 [pubmed]
PHST- 2015/06/06 06:00 [medline]
AID - S1566-0702(14)00099-X [pii]
AID - 10.1016/j.autneu.2014.07.005 [doi]
PST - ppublish
SO  - Auton Neurosci. 2014 Oct;185:67-75. doi: 10.1016/j.autneu.2014.07.005. Epub 2014 
      Jul 17.