PMID- 2509078
OWN - NLM
STAT- MEDLINE
DCOM- 19891207
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 124
IP  - 1
DP  - 1989 Nov
TI  - Effects of cytokines on human thymic epithelial cells in culture: IL1 induces 
      thymic epithelial cell proliferation and change in morphology.
PG  - 13-27
AB  - The role of thymic epithelium in T cell development has given rise to a number of 
      studies, but less information is available concerning the factors regulating 
      thymic epithelial cells (TEC) themselves. Several cytokines, natural or 
      recombinant, were investigated for their effects on human TEC proliferation. This 
      study presents evidence for the first time that human recombinant interleukin 1 
      (IL1) and IL1-containing mixed cytokine preparations induced DNA synthesis of TEC 
      as measured in a 48-hr stimulation assay. The effects of IL1 were dose dependent 
      and sustained in time. The following recombinant cytokines, IL2, IL3, IL4, 
      interferon-gamma (IFN-gamma), IFN-alpha, tumor necrosis factor-alpha (TNF alpha), 
      and TNF beta, as well as thymosin fraction 5 and Escherichia coli 
      lipopolysaccharide (LPS), were not found to modify TEC proliferation but 
      IFN-gamma and TNF alpha enhanced the effects of IL1. We also report that IL1 
      induced a profound change in the morphology of TEC. Our observations suggest that 
      TEC are targets for the action of cytokines and emphasize the important role 
      played by IL1 within the thymus.
FAU - Galy, A H
AU  - Galy AH
AD  - Department of Internal Medicine, University of South Florida College of Medicine, 
      Tampa 33612.
FAU - Hadden, E M
AU  - Hadden EM
FAU - Touraine, J L
AU  - Touraine JL
FAU - Hadden, J W
AU  - Hadden JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Interleukin-1)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Epithelium/drug effects/pathology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/*pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Thymus Gland/*drug effects/pathology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 0008-8749(89)90108-1 [pii]
AID - 10.1016/0008-8749(89)90108-1 [doi]
PST - ppublish
SO  - Cell Immunol. 1989 Nov;124(1):13-27. doi: 10.1016/0008-8749(89)90108-1.