PMID- 25091885
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20211021
IS  - 2737-5935 (Electronic)
IS  - 0037-5675 (Print)
IS  - 0037-5675 (Linking)
VI  - 55
IP  - 7
DP  - 2014 Jul
TI  - Detection of Strongyloides stercoralis infection among cancer patients in a major 
      hospital in Kelantan, Malaysia.
PG  - 367-71
AB  - INTRODUCTION: Strongyloidiasis is one of the most commonly neglected but 
      clinically important parasitic infections worldwide, especially among 
      immunocompromised patients. Evidence of infection among immunocompromised 
      patients in Malaysia is, however, lacking. In this study, microscopy, real-time 
      polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISAs) 
      were used to detect Strongyloides stercoralis (S. stercoralis) infection among 
      cancer patients in a Malaysian hospital. METHODS: A total of 192 stool and serum 
      samples were collected from cancer patients who were receiving chemotherapy with 
      or without steroid treatment at a hospital in northeastern Malaysia. Stool 
      samples were examined for S. stercoralis using parasitological methods and 
      real-time PCR. Serology by ELISA was performed to detect parasite-specific 
      immunoglobulin G (IgG), IgG4 and immunoglobulin E (IgE) antibodies. For 
      comparison, IgG4- and IgG-ELISAs were also performed on the sera of 150 healthy 
      individuals from the same area. RESULTS: Of the 192 samples examined, 1 (0.5%) 
      sample was positive for S. stercoralis by microscopy, 3 (1.6%) by real-time PCR, 
      8 (4.2%) by IgG-ELISA, 6 (3.1%) by IgG4-ELISA, and none was positive by 
      IgE-ELISA. In comparison, healthy blood donors had significantly lower prevalence 
      of parasite-specific IgG (2.67%, p < 0.05) and IgG4 (2.67%, p < 0.05) responses. 
      CONCLUSION: This study showed that laboratory testing may be considered as a 
      diagnostic investigation for S. stercoralis among immunocompromised cancer 
      patients.
FAU - Zueter, AbdelRahman Mohammad
AU  - Zueter AM
FAU - Mohamed, Zeehaida
AU  - Mohamed Z
FAU - Abdullah, Abu Dzarr
AU  - Abdullah AD
FAU - Mohamad, Norsarwany
AU  - Mohamad N
FAU - Arifin, Norsyahida
AU  - Arifin N
FAU - Othman, Nurulhasanah
AU  - Othman N
FAU - Noordin, Rahmah
AU  - Noordin R
AD  - Institute for Research in Molecular Medicine (INFORMM), Universiti Sains 
      Malaysia, 11800 Penang, Malaysia. rahmah8485@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Singapore Med J
JT  - Singapore medical journal
JID - 0404516
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Antibodies, Helminth/blood
MH  - Child
MH  - Child, Preschool
MH  - Cross-Sectional Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Healthy Volunteers
MH  - Hospitalization
MH  - Humans
MH  - Immunocompromised Host
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Malaysia
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/complications/*parasitology
MH  - Real-Time Polymerase Chain Reaction
MH  - *Strongyloides stercoralis
MH  - Strongyloidiasis/blood/*complications/*diagnosis
MH  - Young Adult
PMC - PMC4291962
EDAT- 2014/08/06 06:00
MHDA- 2015/12/17 06:00
PMCR- 2014/07/01
CRDT- 2014/08/06 06:00
PHST- 2014/08/06 06:00 [entrez]
PHST- 2014/08/06 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - SMJ-55-367 [pii]
AID - 10.11622/smedj.2014088 [doi]
PST - ppublish
SO  - Singapore Med J. 2014 Jul;55(7):367-71. doi: 10.11622/smedj.2014088.