PMID- 25092597
OWN - NLM
STAT- MEDLINE
DCOM- 20141009
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1359
DP  - 2014 Sep 12
TI  - Quantification of endogenous brassinosteroids in sub-gram plant tissues by 
      in-line matrix solid-phase dispersion-tandem solid phase extraction coupled with 
      high performance liquid chromatography-tandem mass spectrometry.
PG  - 44-51
LID - S0021-9673(14)01129-7 [pii]
LID - 10.1016/j.chroma.2014.07.037 [doi]
AB  - A matrix solid-phase dispersion (MSPD)-tandem mixed mode anion exchange 
      (MAX)-mixed mode cation exchange (MCX) solid phase extraction-high performance 
      liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) method 
      was developed for quantification of six endogenous brassinosteroids (BRs) 
      (24-epibrassinolide, 24-epicastasterone, 6-deoxo-24-epicastasterone, 
      dolichosterone, teasterone and typhasterol) in rice plant tissues. Non-polar 
      interferences were removed effectively by C8 dispersant used in MSPD, while the 
      following tandem MAX-MCX process facilitated the elimination of polar and 
      ionizable compounds. The weak reversed-phase retention feature of MAX-MCX leaded 
      to good compatibility of the elution solvents in the in-line coupled MSPD-MAX-MCX 
      system. This system was optimized for extraction and purification of BRs in plant 
      samples. The effects of the type of solid phase, the elution solvent, the 
      extraction temperature and the clean-up material were studied. Before HPLC 
      separation, BRs purified were derivatized by m-aminophenylboronic acid to enhance 
      the sensitivity of MS/MS to BRs. Compared with traditional liquid-liquid 
      extraction and solid phase extraction (LLE-SPE), the proposed MSPD-MAX-MCX method 
      showed higher extraction efficiency, lower matrix effect, and advantages of easy 
      manipulation and time-saving. The in-line MSPD-MAX-MCX coupled with HPLC-MS/MS 
      method provided a linear response over two orders of magnitude of BRs 
      concentration with correlation coefficients above 0.9982, limits of detection 
      between 0.008 and 0.04ngmL(-1), relative standard deviations (RSDs) below 29.4%, 
      and recoveries above 77.8%. The proposed method has been successfully applied to 
      analysis of endogenous BRs in rice plant at booting stage and maturity stage.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wang, Lu
AU  - Wang L
AD  - Department of Chemistry, University of Science and Technology of China, Hefei 
      230026, China.
FAU - Duan, Chunfeng
AU  - Duan C
AD  - Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of 
      Sciences, Dalian Institute of Chemical Physics, Dalian 116023, China.
FAU - Wu, Dapeng
AU  - Wu D
AD  - Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of 
      Sciences, Dalian Institute of Chemical Physics, Dalian 116023, China.
FAU - Guan, Yafeng
AU  - Guan Y
AD  - Department of Chemistry, University of Science and Technology of China, Hefei 
      230026, China; Key Laboratory of Separation Science for Analytical Chemistry, 
      Chinese Academy of Sciences, Dalian Institute of Chemical Physics, Dalian 116023, 
      China. Electronic address: guanyafeng@dicp.ac.cn.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140723
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Brassinosteroids)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Brassinosteroids/*chemistry/*isolation & purification
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Molecular Structure
MH  - Oryza/*chemistry
MH  - Plant Growth Regulators/*chemistry/*isolation & purification
MH  - Solid Phase Extraction/instrumentation/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Brassinosteroids
OT  - Derivatization
OT  - In-line matrix solid phase dispersion-tandem solid phase extraction
OT  - Mixed mode anion exchange-mixed mode cation exchange
OT  - Rice plant
EDAT- 2014/08/06 06:00
MHDA- 2014/10/10 06:00
CRDT- 2014/08/06 06:00
PHST- 2014/04/15 00:00 [received]
PHST- 2014/06/13 00:00 [revised]
PHST- 2014/07/13 00:00 [accepted]
PHST- 2014/08/06 06:00 [entrez]
PHST- 2014/08/06 06:00 [pubmed]
PHST- 2014/10/10 06:00 [medline]
AID - S0021-9673(14)01129-7 [pii]
AID - 10.1016/j.chroma.2014.07.037 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Sep 12;1359:44-51. doi: 10.1016/j.chroma.2014.07.037. Epub 
      2014 Jul 23.