PMID- 25093862
OWN - NLM
STAT- MEDLINE
DCOM- 20150424
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 8
DP  - 2014
TI  - Green tea extract treatment alleviates ocular inflammation in a rat model of 
      endotoxin-induced uveitis.
PG  - e103995
LID - 10.1371/journal.pone.0103995 [doi]
LID - e103995
AB  - Green tea extract (GTE) ingested by rats exerted anti-oxidative activities in 
      various ocular tissues as shown in our previous studies. The present work 
      investigated anti-inflammatory effects of GTE on endotoxin-induced uveitis (EIU). 
      EIU was generated in adult rats by a footpad injection of 1 mg/kg 
      lipopolysaccharide (LPS). Oral administration of GTE (550 mg/kg) was given one, 
      two or four times after LPS injection. Twenty-four hours later, LPS produced 
      severe hyperemia and edema in the iris. Immunocytochemical examinations showed an 
      accumulation of infiltrating cells in the aqueous humor that were immunopositive 
      for cluster of differentiation 43 (CD43) and CD68, markers for leucocytes and 
      macrophages, respectively. Analyses of the aqueous humor showed an increase in 
      pro-inflammatory mediators including tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). GTE 
      treatments improved the clinical manifestations and reduced infiltrating cells 
      and protein exudation in the aqueous humor, which were not observed under half 
      dose of GTE (275 mg/kg). The number of CD68 positive macrophages residing in the 
      iris and ciliary was also reduced. GTE suppressed production of TNF-alpha, IL-6 and 
      MCP-1 in the aqueous humor, which was associated with a down-regulation of LPS 
      receptor complex subunits, Toll-like receptor 4 (TLR-4) and CD14, and suppression 
      of nuclear factor-kappa Bp65 (NF-kappaBp65) in the iris and ciliary body. Our 
      findings show that GTE is a potent anti-inflammatory agent against the 
      inflammation of EIU, and suggest a potential use in treatment of acute uveitis.
FAU - Qin, Yong Jie
AU  - Qin YJ
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Chu, Kai On
AU  - Chu KO
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Yip, Yolanda Wong Ying
AU  - Yip YW
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Li, Wai Ying
AU  - Li WY
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Yang, Ya Ping
AU  - Yang YP
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Chan, Kwok Ping
AU  - Chan KP
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
FAU - Ren, Jia Lin
AU  - Ren JL
AD  - School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Chan, Sun On
AU  - Chan SO
AD  - School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Pang, Chi Pui
AU  - Pang CP
AD  - Department of Ophthalmology and Visual Sciences, The Chinese University of Hong 
      Kong, Hong Kong.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140805
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
SB  - IM
MH  - Animals
MH  - Aqueous Humor/cytology/immunology
MH  - Catechin/analogs & derivatives/pharmacology
MH  - Disease Models, Animal
MH  - Endotoxins
MH  - Inflammation/chemically induced/drug therapy
MH  - Lipopolysaccharides
MH  - Neutrophil Infiltration
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tea/chemistry
MH  - Uveitis/chemically induced/*drug therapy
PMC - PMC4122397
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/08/06 06:00
MHDA- 2015/04/25 06:00
PMCR- 2014/08/05
CRDT- 2014/08/06 06:00
PHST- 2014/01/15 00:00 [received]
PHST- 2014/07/06 00:00 [accepted]
PHST- 2014/08/06 06:00 [entrez]
PHST- 2014/08/06 06:00 [pubmed]
PHST- 2015/04/25 06:00 [medline]
PHST- 2014/08/05 00:00 [pmc-release]
AID - PONE-D-14-02072 [pii]
AID - 10.1371/journal.pone.0103995 [doi]
PST - epublish
SO  - PLoS One. 2014 Aug 5;9(8):e103995. doi: 10.1371/journal.pone.0103995. eCollection 
      2014.