PMID- 2509611
OWN - NLM
STAT- MEDLINE
DCOM- 19891204
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 46
IP  - 5
DP  - 1989 Nov
TI  - Gamma interferon induces monocyte killing of Listeria monocytogenes by an 
      oxygen-dependent pathway; alpha- or beta-interferons by oxygen-independent 
      pathways.
PG  - 434-40
AB  - Human peripheral blood monocytes purified by counterflow centrifugal elutriation 
      were treated with recombinant interferons-gamma (IFN-gamma), alpha (IFN-alpha), 
      or beta (IFN-beta)--and tested for their capacity to kill Listeria monocytogenes. 
      All three IFNs increased the monocyte bactericidal activity in a dose-dependent 
      fashion. Exogeneous catalase, an inhibitor of monocyte-generated hydrogen 
      peroxide, did not affect bactericidal activity. However, exogenous superoxide 
      dismutase inhibited killing by IFN-gamma-activated monocytes, but not by 
      IFN-alpha- or IFN-beta-activated monocytes. By contrast, exogenous soybean 
      trypsin inhibitor inhibited killing by IFN-alpha or IFN-beta-activated monocytes, 
      but not by IFN-gamma-activated monocytes. Combinations of IFN-gamma and IFN-alpha 
      resulted in no increase in bactericidal activity. Finally, treatment with 
      IFN-gamma resulted in different receptiveness of the cell to subsequent oxidative 
      burst-stimulating signals than did treatment with either IFN-alpha or IFN-beta. 
      These results suggest that monocytes treated with IFN-gamma kill L. monocytogenes 
      by an oxygen-dependent mechanism, but treatment with IFN-alpha or IFN-beta 
      elicits principally oxygen-independent mechanisms.
FAU - Peck, R
AU  - Peck R
AD  - Central Research Unit, F. Hoffmann-La Roche & Co., Ltd., Basel, Switzerland.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Free Radicals)
RN  - 0 (Interferon Type I)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Blood Bactericidal Activity/*drug effects
MH  - Free Radicals
MH  - Humans
MH  - Interferon Type I/*pharmacology
MH  - Interferon-gamma/*pharmacology
MH  - Listeria monocytogenes/*immunology
MH  - Macrophages/immunology
MH  - Monocytes/*immunology
MH  - Oxygen/*metabolism
MH  - Recombinant Proteins
MH  - Superoxide Dismutase/pharmacology
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 10.1002/jlb.46.5.434 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1989 Nov;46(5):434-40. doi: 10.1002/jlb.46.5.434.