PMID- 25098936
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20140807
IS  - 1435-1803 (Electronic)
IS  - 0300-8428 (Linking)
VI  - 109
IP  - 5
DP  - 2014
TI  - Multifunctional MR monitoring of the healing process after myocardial infarction.
PG  - 430
LID - 10.1007/s00395-014-0430-0 [doi]
AB  - Healing of the myocardium after infarction comprises a variety of local adaptive 
      processes which contribute to the functional outcome after the insult. Therefore, 
      we aimed to establish a setting for concomitant assessment of regional 
      alterations in contractile function, morphology, and immunological state to gain 
      prognostic information on cardiac recovery after infarction. For this, mice were 
      subjected to myocardial ischemia/reperfusion (I/R) and monitored for 28 days by 
      cine MRI, T2 mapping, late gadolinium enhancement (LGE), and (19)F MRI. T2 values 
      were calculated from gated multi-echo sequences. (19)F-loaded nanoparticles were 
      injected intravenously for labelling circulating monocytes and making them 
      detectable by (19)F MRI. In-house developed software was used for regional 
      analysis of cine loops, T2 maps, LGE, and (19)F images to correlate local wall 
      movement, tissue damage as well as monocyte recruitment over up to 200 sectors 
      covering the left ventricle. This enabled us to evaluate simultaneously zonal 
      cardiac necrosis, oedema, and inflammation patterns together with sectional 
      fractional shortening (FS) and global myocardial function. Oedema, indicated by a 
      rise in T2, showed a slightly better correlation with FS than LGE. Regional T2 
      values increased from 19 ms to above 30 ms after I/R. In the course of the 
      healing process oedema resolved within 28 days, while myocardial function 
      recovered. Infiltrating monocytes could be quantitatively tracked by (19)F MRI, 
      as validated by flow cytometry. Furthermore, (19)F MRI proved to yield valuable 
      insight on the outcome of myocardial infarction in a transgenic mouse model. In 
      conclusion, our approach permits a comprehensive surveillance of key processes 
      involved in myocardial healing providing independent and complementary 
      information for individual prognosis.
FAU - Bonner, Florian
AU  - Bonner F
AD  - Department of Molecular Cardiology, Heinrich Heine University Dusseldorf, 
      Universitatsstr. 1, 40225, Dusseldorf, Germany.
FAU - Jacoby, Christoph
AU  - Jacoby C
FAU - Temme, Sebastian
AU  - Temme S
FAU - Borg, Nadine
AU  - Borg N
FAU - Ding, Zhaoping
AU  - Ding Z
FAU - Schrader, Jurgen
AU  - Schrader J
FAU - Flogel, Ulrich
AU  - Flogel U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140807
PL  - Germany
TA  - Basic Res Cardiol
JT  - Basic research in cardiology
JID - 0360342
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Image Interpretation, Computer-Assisted/*methods
MH  - Magnetic Resonance Imaging/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Myocardial Infarction/*pathology
MH  - Wound Healing/*physiology
EDAT- 2014/08/08 06:00
MHDA- 2015/02/24 06:00
CRDT- 2014/08/08 06:00
PHST- 2014/02/11 00:00 [received]
PHST- 2014/07/28 00:00 [accepted]
PHST- 2014/07/22 00:00 [revised]
PHST- 2014/08/08 06:00 [entrez]
PHST- 2014/08/08 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
AID - 10.1007/s00395-014-0430-0 [doi]
PST - ppublish
SO  - Basic Res Cardiol. 2014;109(5):430. doi: 10.1007/s00395-014-0430-0. Epub 2014 Aug 
      7.