PMID- 25099764
OWN - NLM
STAT- MEDLINE
DCOM- 20150402
LR  - 20220331
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Linking)
VI  - 31
IP  - 9
DP  - 2014 Sep
TI  - Combined detection of serum exosomal miR-21 and HOTAIR as diagnostic and 
      prognostic biomarkers for laryngeal squamous cell carcinoma.
PG  - 148
LID - 10.1007/s12032-014-0148-8 [doi]
AB  - Serum exosomes containing noncoding RNA (ncRNA) play an important role in both 
      physiological and pathological conditions. However, biological function of 
      exosomal ncRNA remains unclear. The aim of the study was to investigate the 
      prognostic and diagnostic values of exosomal ncRNA by comparing the amounts of 
      exosomal miR-21 and HOTAIR in serum of laryngeal squamous cell carcinoma (LSCC) 
      patients with those of polyps of vocal cords, and by determinating whether 
      combined detection of the two molecules could provide useful information in the 
      diagnosis of LSCC. Exosomes were isolated from the serum samples of 52 LSCC 
      patients and those of 49 patients with polyps of vocal cords. TEM and Western 
      blot were applied for the confirmation of isolated exosomes by observing the 
      ultra structure and testing CD63 marker protein, respectively. RT-PCR was 
      performed to detect the expression of miR-21 and HOTAIR in the exosomes. The 
      receiver-operating characteristic (ROC) curve was generated to examine the 
      prognostic value of the two molecules. The expression of exosomal miR-21 and 
      HOTAIR was significantly higher in patients with LSCC than those with vocal cord 
      polyps. There were significant differences of serum exosomal miR-21 and HOTAIR 
      expressions between the advanced T classifications (T3/T4) or clinical stages 
      (III/IV) and the early stages. The patients with lymph node metastasis had higher 
      serum exosomal miR-21 and HOTAIR expressions than those without. There were no 
      differences between patient sex, tumor locations and differentiations. The area 
      under the ROC curve of combined examination of exosomal HOTAIR and miR-21 for 
      diagnosing LSCC was 87.6 %, which was significantly higher than 80.1 % of miR-21 
      (p = 0.0359) or 72.7 % of HOTAIR (p = 0.0012), showing 94.2 and 73.5 % of 
      sensitivity and specificity, respectively, in differentiating the malignant from 
      benign laryngeal disease. Serum exosomal miR-21 and HOTAIR were significantly 
      correlated with clinical parameters of LSCC, and combined evaluation of their 
      serum expressions may be a valuable biomarker to screen LSCC and might be a 
      promising predicting tool for LSCC patient.
FAU - Wang, Jingting
AU  - Wang J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Zhou, Yandong
AU  - Zhou Y
FAU - Lu, Jianguang
AU  - Lu J
FAU - Sun, Yanan
AU  - Sun Y
FAU - Xiao, Hui
AU  - Xiao H
FAU - Liu, Ming
AU  - Liu M
FAU - Tian, Linli
AU  - Tian L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140807
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Biomarkers, Tumor/*blood
MH  - Carcinoma, Squamous Cell/blood/*diagnosis/epidemiology
MH  - Cohort Studies
MH  - Exosomes/*chemistry
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/blood/*diagnosis/epidemiology
MH  - Male
MH  - MicroRNAs/*blood
MH  - Middle Aged
MH  - Polyps/blood/diagnosis/epidemiology
MH  - Prognosis
MH  - RNA, Long Noncoding/*blood
MH  - ROC Curve
EDAT- 2014/08/08 06:00
MHDA- 2015/04/04 06:00
CRDT- 2014/08/08 06:00
PHST- 2014/06/24 00:00 [received]
PHST- 2014/07/26 00:00 [accepted]
PHST- 2014/08/08 06:00 [entrez]
PHST- 2014/08/08 06:00 [pubmed]
PHST- 2015/04/04 06:00 [medline]
AID - 10.1007/s12032-014-0148-8 [doi]
PST - ppublish
SO  - Med Oncol. 2014 Sep;31(9):148. doi: 10.1007/s12032-014-0148-8. Epub 2014 Aug 7.