PMID- 25101883
OWN - NLM
STAT- MEDLINE
DCOM- 20150804
LR  - 20141229
IS  - 1549-9642 (Electronic)
IS  - 1549-9634 (Linking)
VI  - 11
IP  - 1
DP  - 2015 Jan
TI  - Virus-like particle-mediated intracellular delivery of mRNA cap analog with in 
      vivo activity against hepatocellular carcinoma.
PG  - 67-76
LID - S1549-9634(14)00419-5 [pii]
LID - 10.1016/j.nano.2014.07.009 [doi]
AB  - Adenovirus dodecahedron (Dd), a nanoparticulate proteinaceous biodegradable 
      virus-like particle (VLP), was used as a vector for delivery of an oncogene 
      inhibitor to hepatocellular carcinoma (HCC) rat orthotopic model. Initiation 
      factor eIF4E is an oncogene with elevated expression in human cancers. 
      Cell-impermeant eIF4E inhibitor, cap structure analog (cap) and anti-cancer 
      antibiotic doxorubicin (Dox) were delivered as Dd conjugates. Dd-cap and Dd-dox 
      inhibited cancer cell culture proliferation up to 50 and 84%, respectively, while 
      with free Dox similar results could be obtained only at a 5 times higher 
      concentration. In animal HCC model the combination treatment of Dd-cap/Dd-dox 
      caused 40% inhibition of tumor growth. Importantly, the level of two 
      pro-oncogenes, eIF4E and c-myc, was significantly diminished in tumor sections of 
      treated rats. Attachment to Dd, a virus-like particle, permitted the first 
      demonstration of cap analog intracellular delivery and resulted in improved 
      doxorubicin delivery leading to statistically significant inhibition of HCC tumor 
      growth.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Zochowska, Monika
AU  - Zochowska M
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, 
      Poland.
FAU - Piguet, Anne-Christine
AU  - Piguet AC
AD  - Hepatology, Department of Clinical Research, University of Berne, Berne, 
      Switzerland.
FAU - Jemielity, Jacek
AU  - Jemielity J
AD  - Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, 
      University of Warsaw, Warsaw, Poland; Centre of New Technologies, University of 
      Warsaw, Warsaw, Poland.
FAU - Kowalska, Joanna
AU  - Kowalska J
AD  - Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, 
      University of Warsaw, Warsaw, Poland.
FAU - Szolajska, Ewa
AU  - Szolajska E
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, 
      Poland.
FAU - Dufour, Jean-Francois
AU  - Dufour JF
AD  - University Clinics of Visceral Surgery and Medicine, Inselspital Berne, Berne, 
      Switzerland.
FAU - Chroboczek, Jadwiga
AU  - Chroboczek J
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, 
      Poland; Therex, TIMC-IMAG, CNRS, Universite Joseph Fourier, La Tronche, France. 
      Electronic address: jadwiga.chroboczek@imag.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140805
PL  - United States
TA  - Nanomedicine
JT  - Nanomedicine : nanotechnology, biology, and medicine
JID - 101233142
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vaccines, Virus-Like Particle)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Adenoviridae/metabolism
MH  - Animals
MH  - Antineoplastic Agents/chemistry
MH  - Carcinoma, Hepatocellular/*therapy
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Cell Proliferation
MH  - Cytoplasm/metabolism
MH  - Doxorubicin/chemistry
MH  - Endocytosis
MH  - Eukaryotic Initiation Factor-4E/*metabolism
MH  - Genetic Vectors
MH  - HeLa Cells
MH  - Humans
MH  - Liver Neoplasms/*therapy
MH  - Nanomedicine/methods
MH  - Neoplasm Transplantation
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Vaccines, Virus-Like Particle/*pharmacology
OTO - NOTNLM
OT  - Adenoviral dodecahedron
OT  - Cap structure analogs
OT  - Doxorubicin
OT  - Drug delivery vector
OT  - Hepatocellular carcinoma
OT  - Oncogene eIF4E
OT  - Virus-like particle
OT  - eIF4E inhibitor
EDAT- 2014/08/08 06:00
MHDA- 2015/08/05 06:00
CRDT- 2014/08/08 06:00
PHST- 2013/11/06 00:00 [received]
PHST- 2014/06/17 00:00 [revised]
PHST- 2014/07/23 00:00 [accepted]
PHST- 2014/08/08 06:00 [entrez]
PHST- 2014/08/08 06:00 [pubmed]
PHST- 2015/08/05 06:00 [medline]
AID - S1549-9634(14)00419-5 [pii]
AID - 10.1016/j.nano.2014.07.009 [doi]
PST - ppublish
SO  - Nanomedicine. 2015 Jan;11(1):67-76. doi: 10.1016/j.nano.2014.07.009. Epub 2014 
      Aug 5.