PMID- 2510238
OWN - NLM
STAT- MEDLINE
DCOM- 19891215
LR  - 20191022
IS  - 0172-8172 (Print)
IS  - 0172-8172 (Linking)
VI  - 9
IP  - 2
DP  - 1989
TI  - Comparative and combined effects of transforming growth factors alpha and beta, 
      interleukin-1 and interferon-gamma on rheumatoid synovial cell proliferation, 
      glycolysis and prostaglandin E production.
PG  - 65-70
AB  - Enhanced cell proliferation, glycolysis and prostaglandin E production are all 
      characteristic features of rheumatoid synovial tissue. The interrelationships of 
      these three cellular parameters have been examined using rheumatoid synovial 
      fibroblasts and their responses to specific cytokines in vitro. Transforming 
      growth factor alpha (TGF alpha) caused a more than threefold increase in synovial 
      cell proliferation whilst transforming growth factor beta (TGF beta), 
      interleukin-1 alpha (IL-1 alpha) and interferon-gamma (IFN-gamma) produced only 
      marginal changes. The combined addition of IL-1 alpha with TGF beta resulted in 
      an enhanced proliferative response comparable with that produced by TGF alpha. 
      Glycolysis, estimated by glucose utilisation and measurements of the glycolytic 
      regulatory metabolite fructose 2,6-bisphosphate was significantly stimulated by 
      TGF beta, IL-1 alpha and IFN-gamma, but less so by TGF alpha. Prostaglandin E 
      production was significantly increased by IL-1 alpha to an extent much greater 
      than that produced by TGF alpha or TGF beta, although the combined addition of 
      IL-1 alpha with either TGF alpha or beta resulted in a synergistic increase in 
      PGE production, a response partly diminished by the addition of IFN-gamma. These 
      findings suggest that the extent to which a cytokine stimulates glycolysis is not 
      consistently related to its mitogenicity, and that cytokine combinations which 
      stimulate high levels of PGE production (a growth inhibitor) will not necessarily 
      be associated with a reduced rate of cellular proliferation in cultured, 
      adherent, rheumatoid synovial fibroblasts.
FAU - Taylor, D J
AU  - Taylor DJ
AD  - University Department of Medicine, University Hospital of South Manchester, West 
      Didsbury, UK.
FAU - Feldmann, M
AU  - Feldmann M
FAU - Evanson, J M
AU  - Evanson JM
FAU - Woolley, D E
AU  - Woolley DE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Biological Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Prostaglandins E)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Biological Factors/*pharmacology
MH  - Cell Division/drug effects
MH  - Cytokines
MH  - Glycolysis/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Prostaglandins E/biosynthesis
MH  - Rheumatic Diseases/metabolism/*pathology
MH  - Synovial Membrane/*drug effects/metabolism/pathology
MH  - Transforming Growth Factors/pharmacology
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1007/BF00270247 [doi]
PST - ppublish
SO  - Rheumatol Int. 1989;9(2):65-70. doi: 10.1007/BF00270247.