PMID- 25102917
OWN - NLM
STAT- MEDLINE
DCOM- 20141216
LR  - 20140929
IS  - 1217-8950 (Print)
IS  - 1217-8950 (Linking)
VI  - 61
IP  - 3
DP  - 2014 Sep
TI  - Prevalence of human herpesvirus 6A and 6B during pregnancy.
PG  - 309-16
LID - 10.1556/AMicr.61.2014.3.5 [doi]
AB  - The aim of the present study was to assess the frequency of human herpesvirus 6A 
      (HHV-6A) and human herpesvirus 6B (HHV-6B) infection during pregnancy. 100-100 
      blood samples were collected from pregnant and non-pregnant women, then nucleic 
      acid was isolated from both plasma and leukocytes fraction. Nested and real-time 
      PCR were used to detect and differentiate HHV-6A and HHV-6B DNA and to determine 
      viral loads. Reverse transcription PCR (RT-PCR) for HHV-6 U79/80 mRNA was 
      performed in order to reveal active HHV-6 replication.HHV-6A and HHV-6B active 
      infections were not detected in blood samples neither from pregnant nor from 
      non-pregnant women. Frequency of HHV-6B and HHV-6A latency did not show 
      difference between the studied groups (15% vs. 16%). HHV-6B latency was dominant 
      in both studied groups (14/15 and 15/16). Beside these results, in leukocyte 
      samples of one pregnant and three non-pregnant women high HHV-6A viral loads 
      (1.28 x 10(5) - 5.07 x 10(5) GEq / 1.5 x 10(6) leukocytes) were detected, and viral DNA 
      was also found in plasma samples. Although RT-PCR did not confirm virus 
      replication, but chromosomal integration was also not proved unequivocally, the 
      number of 0.08-0.33 HHV-6 copy / 1 leukocyte refers more to postnatal infection.
FAU - Meszaros, Beata
AU  - Meszaros B
AD  - University of Debrecen Department of Medical Microbiology Debrecen Hungary.
FAU - Sapy, Tamas
AU  - Sapy T
AD  - University of Debrecen Department of Obstetrics and Gynaecology Debrecen Hungary.
FAU - Gergely, Lajos
AU  - Gergely L
AD  - University of Debrecen Department of Medical Microbiology Debrecen Hungary.
FAU - Csoma, Eszter
AU  - Csoma E
AD  - University of Debrecen Department of Medical Microbiology Debrecen Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Microbiol Immunol Hung
JT  - Acta microbiologica et immunologica Hungarica
JID - 9434021
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - DNA, Viral/genetics
MH  - Female
MH  - Herpesvirus 6, Human/classification/genetics/*isolation & purification/physiology
MH  - Humans
MH  - Hungary/epidemiology
MH  - Pregnancy
MH  - Pregnancy Complications/*epidemiology/virology
MH  - Prevalence
MH  - Roseolovirus Infections/*epidemiology/virology
MH  - Viral Load
MH  - Young Adult
OTO - NOTNLM
OT  - HHV-6A
OT  - HHV-6B
OT  - PCR
OT  - blood
OT  - healthy women
OT  - pregnancy
EDAT- 2014/08/12 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/08/09 06:00
PHST- 2014/08/09 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - H104162J54318716 [pii]
AID - 10.1556/AMicr.61.2014.3.5 [doi]
PST - ppublish
SO  - Acta Microbiol Immunol Hung. 2014 Sep;61(3):309-16. doi: 
      10.1556/AMicr.61.2014.3.5.