PMID- 25103256
OWN - NLM
STAT- MEDLINE
DCOM- 20150703
LR  - 20211021
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 18
IP  - 11
DP  - 2014 Nov
TI  - Shear flow affects selective monocyte recruitment into MCP-1-loaded scaffolds.
PG  - 2176-88
LID - 10.1111/jcmm.12330 [doi]
AB  - Novel cardiovascular replacements are being developed by using degradable 
      synthetic scaffolds, which function as a temporary guide to induce neotissue 
      formation directly in situ. Priming of such scaffolds with fast-releasing 
      monocyte chemoattractant protein-1 (MCP-1) was shown to improve the formation of 
      functional neoarteries in rats. However, the underlying mechanism has not been 
      clarified. Therefore, the goal of this study was to investigate the effect of a 
      burst-release of MCP-1 from a synthetic scaffold on the local recruitment of 
      circulating leucocytes under haemodynamic conditions. Herein, we hypothesized 
      that MCP-1 initiates a desired healing cascade by recruiting favourable monocyte 
      subpopulations into the implanted scaffold. Electrospun poly(epsilon-caprolactone) 
      scaffolds were loaded with fibrin gel containing various doses of MCP-1 and 
      exposed to a suspension of human peripheral blood mononuclear cells in static or 
      dynamic conditions. In standard migration assay, a dose-dependent migration of 
      specific CD14(+) monocyte subsets was observed, as measured by flow cytometry. In 
      conditions of pulsatile flow, on the other hand, a marked increase in immediate 
      monocyte recruitment was observed, but without evident selectivity in monocyte 
      subsets. This suggests that the selectivity was dependent on the release kinetics 
      of the MCP-1, as it was overruled by the effect of shear stress after the initial 
      burst-release. Furthermore, these findings demonstrate that local recruitment of 
      specific MCP-1-responsive monocytes is not the fundamental principle behind the 
      improved neotissue formation observed in long-term in vivo studies, and 
      mobilization of MCP-1-responsive cells from the bone marrow into the bloodstream 
      is suggested to play a predominant role in vivo.
CI  - (c) 2014 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Smits, Anthal I P M
AU  - Smits AI
AD  - Soft Tissue Biomechanics and Tissue Engineering, Department of Biomedical 
      Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
FAU - Ballotta, Virginia
AU  - Ballotta V
FAU - Driessen-Mol, Anita
AU  - Driessen-Mol A
FAU - Bouten, Carlijn V C
AU  - Bouten CV
FAU - Baaijens, Frank P T
AU  - Baaijens FP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140808
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
SB  - IM
MH  - Absorbable Implants
MH  - Animals
MH  - Cell Count
MH  - Cells, Cultured
MH  - Chemokine CCL2/chemistry/*metabolism
MH  - Coronary Vessels/cytology/*growth & development/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/*cytology/metabolism
MH  - Male
MH  - Polyesters/chemistry
MH  - Rats
MH  - *Tissue Scaffolds
PMC - PMC4224552
OTO - NOTNLM
OT  - CCL2
OT  - angiogenic monocytes
OT  - bioactive scaffold
OT  - cardiovascular
OT  - chemotaxis
OT  - haemodynamics
OT  - immunomodulation
OT  - in situ tissue engineering
OT  - peripheral blood mononuclear cells
EDAT- 2014/08/12 06:00
MHDA- 2015/07/04 06:00
PMCR- 2014/11/01
CRDT- 2014/08/09 06:00
PHST- 2013/12/10 00:00 [received]
PHST- 2014/04/23 00:00 [accepted]
PHST- 2014/08/09 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/07/04 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - 10.1111/jcmm.12330 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2014 Nov;18(11):2176-88. doi: 10.1111/jcmm.12330. Epub 2014 Aug 
      8.