PMID- 25105257
OWN - NLM
STAT- MEDLINE
DCOM- 20151021
LR  - 20220408
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 76
IP  - 5
DP  - 2014 Nov
TI  - Intra-amniotic LPS modulates expression of antimicrobial peptides in the fetal 
      sheep lung.
PG  - 441-7
LID - 10.1038/pr.2014.113 [doi]
AB  - BACKGROUND: Damage-associated molecular patterns (DAMPs) and antimicrobial 
      peptides (AMPs) are components of pulmonary innate immunity and tissue repair. We 
      hypothesized that DAMPs and AMPs would increase in response to fetal pulmonary 
      inflammation caused by chorioamnionitis in a time-dependent manner. METHODS: 
      Fetal sheep were exposed to intra-amniotic saline or lipopolysaccharide (LPS) 
      (10 mg) between 5 h and 15 d prior to preterm delivery at 125 +/- 2 d. Lung tissue 
      mRNAs for proinflammatory cytokines; AMPs: myeloid AMP-29 (MAP29), dodecapeptide, 
      sheep beta-defensin-1 (SBD1), and sheep beta-defensin-2 (SBD2); and DAMPs: interleukin 
      (IL)-1alpha, lactoferrin, heat-shock protein-70 (HSP70), high-mobility group box 
      protein-B1 (HMGB1), and receptor for advanced glycation endproducts (RAGE) were 
      measured by reverse-transcriptase quantitative polymerase chain reaction. 
      Immunohistochemistry of DAMPs and in situ hybridization of AMPs was performed. 
      RESULTS: IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, MCP-1, and tumor necrosis factor 
      (TNF)-alpha mRNA increased after LPS exposure. MAP29, dodecapeptide, SBD1, and SBD2 
      mRNA were suppressed at 24 h. MAP29 and dodecapeptide mRNA then increased at 8 d. 
      Lactoferrin increased at 24 h. There were no changes for HMGB1, HSP70, or RAGE. 
      MAP29 and dodecapeptide localized to alveolar cells, increased 8 d after exposure 
      to LPS. CONCLUSION: AMPs are initially suppressed in the fetal lung by 
      LPS-induced chorioamnionitis. The late induction of MAP29 and dodecapeptide may 
      be related to lung repair.
FAU - Schmidt, Augusto F
AU  - Schmidt AF
AD  - Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 
      University of Cincinnati, Cincinnati, Ohio.
FAU - Kannan, Paranthaman S
AU  - Kannan PS
AUID- ORCID: 0000000233333720
AD  - Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 
      University of Cincinnati, Cincinnati, Ohio.
FAU - Kemp, Matthew W
AU  - Kemp MW
AD  - School of Women's and Infants' Health, The University of Western Australia, 
      Perth, Australia.
FAU - Kramer, Boris W
AU  - Kramer BW
AD  - Department of Pediatrics, Maastricht University Medical Center, Maastricht, The 
      Netherlands.
FAU - Newnham, John P
AU  - Newnham JP
AD  - School of Women's and Infants' Health, The University of Western Australia, 
      Perth, Australia.
FAU - Jobe, Alan H
AU  - Jobe AH
AD  - Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 
      University of Cincinnati, Cincinnati, Ohio.
FAU - Kallapur, Suhas G
AU  - Kallapur SG
AD  - Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 
      University of Cincinnati, Cincinnati, Ohio.
LA  - eng
GR  - R01 HD057869/HD/NICHD NIH HHS/United States
GR  - HD-57869/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140808
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amniotic Fluid/*metabolism
MH  - Animals
MH  - Antimicrobial Cationic Peptides/genetics/*metabolism
MH  - Chorioamnionitis/chemically induced/genetics/*metabolism
MH  - Cytokines/genetics/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation
MH  - Gestational Age
MH  - Inflammation Mediators/metabolism
MH  - *Lipopolysaccharides
MH  - Lung/growth & development/*metabolism
MH  - Pregnancy
MH  - Premature Birth
MH  - RNA, Messenger/metabolism
MH  - Sheep
MH  - Time Factors
PMC - PMC4213214
MID - NIHMS598649
EDAT- 2014/08/12 06:00
MHDA- 2015/10/22 06:00
PMCR- 2015/05/01
CRDT- 2014/08/09 06:00
PHST- 2013/11/22 00:00 [received]
PHST- 2014/05/11 00:00 [accepted]
PHST- 2014/08/09 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/10/22 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - pr2014113 [pii]
AID - 10.1038/pr.2014.113 [doi]
PST - ppublish
SO  - Pediatr Res. 2014 Nov;76(5):441-7. doi: 10.1038/pr.2014.113. Epub 2014 Aug 8.