PMID- 25105597
OWN - NLM
STAT- MEDLINE
DCOM- 20151001
LR  - 20161125
IS  - 1557-8550 (Electronic)
IS  - 1549-5418 (Linking)
VI  - 32
IP  - 9
DP  - 2014 Sep
TI  - Phototoxic effect of na-pheophorbide a toward osteosarcoma cells in vitro using a 
      laser diode.
PG  - 481-9
LID - 10.1089/pho.2014.3736 [doi]
AB  - OBJECTIVE: The purpose of this study was to investigate the effectiveness of 
      photodynamic therapy (PDT) with Na-pheophorbide A in anticancer treatment, using 
      osteosarcoma cells in vitro. BACKGROUND DATA: It has been reported that PDT with 
      chlorophyll derivatives inhibits the proliferation of various cancer cells. 
      However, there have been no reports that have evaluated the effectiveness of PDT 
      in suppressing osteosarcoma cells. MATERIALS AND METHODS: Uptake of 
      Na-pheophorbide A into Hu09 cells (osteosarcoma cells) was assayed using 
      fluorescence microscopy following incubation of the cells with 28 mumol/L of 
      Na-pheophorbide A. The viability of Hu09 cells after PDT treatment was assessed 
      using trypan blue dye staining and MTS assays. PDT-induced apoptosis was 
      determined by evaluation of the activity of selected members of the caspase 
      family and by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining of cells. RESULTS: Na-pheophorbide A uptake by cells 
      was rapid, being observed after 60 min of treatment, and Na-pheophorbide A 
      persisted in cells for >24 h. PDT treatment decreased cell viability compared 
      with the control group, indicating high cytocidal activity of PDT. This cytocidal 
      effect was dependent upon drug concentration, light dose, and the number of 
      irradiation times. An increase in the number of cells positive for TUNEL staining 
      and increases in the activity of caspases-3, -8 and -9 were observed in the first 
      2 h after PDT treatment. CONCLUSIONS: A cytotoxic effect of PDT with 
      Na-pheophorbide A on an osteosarcoma cell line in vitro was shown. Caspase 
      activity assays suggested that PDT with Na-pheophorbide A induced an apoptotic 
      change in HuO9 cells, mainly via activation of mitochondrial caspase -9 and -3 
      pathways.
FAU - Nagai, Yu
AU  - Nagai Y
AD  - 1 Department of Orthopedic Surgery, Surugadai Nihon University Hospital , Tokyo, 
      Japan .
FAU - Aizawa, Shin
AU  - Aizawa S
FAU - Iriuchishima, Takanori
AU  - Iriuchishima T
FAU - Goto, Bunsei
AU  - Goto B
FAU - Nagaoka, Masahiro
AU  - Nagaoka M
FAU - Tokuhashi, Yasuaki
AU  - Tokuhashi Y
FAU - Saito, Akiyoshi
AU  - Saito A
LA  - eng
PT  - Journal Article
DEP - 20140808
PL  - United States
TA  - Photomed Laser Surg
JT  - Photomedicine and laser surgery
JID - 101222340
RN  - 0 (Radiation-Sensitizing Agents)
RN  - 1406-65-1 (Chlorophyll)
RN  - EC 3.4.22.- (Caspase 3)
RN  - IA2WNI2HO2 (pheophorbide a)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Bone Neoplasms/*drug therapy
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival
MH  - Chlorophyll/*analogs & derivatives/pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - In Vitro Techniques
MH  - *Lasers, Semiconductor
MH  - Microscopy, Fluorescence
MH  - Osteosarcoma/*drug therapy
MH  - Photochemotherapy/*methods
MH  - Radiation-Sensitizing Agents/*pharmacology
EDAT- 2014/08/12 06:00
MHDA- 2015/10/02 06:00
CRDT- 2014/08/09 06:00
PHST- 2014/08/09 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/10/02 06:00 [medline]
AID - 10.1089/pho.2014.3736 [doi]
PST - ppublish
SO  - Photomed Laser Surg. 2014 Sep;32(9):481-9. doi: 10.1089/pho.2014.3736. Epub 2014 
      Aug 8.