PMID- 25106037
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20181202
IS  - 1573-2517 (Electronic)
IS  - 0165-0327 (Linking)
VI  - 168
DP  - 2014 Oct
TI  - Brain-derived neurotrophic factor (BDNF)-epigenetic regulation in unipolar and 
      bipolar affective disorder.
PG  - 399-406
LID - S0165-0327(14)00453-4 [pii]
LID - 10.1016/j.jad.2014.07.022 [doi]
AB  - BACKGROUND: Alterations of brain-derived neurotrophic factor (BDNF) DNA 
      methylation at specific BDNF promoters and corresponding gene expressions are 
      associated with pathology and the response to antidepressant (AD) therapy in 
      affective disorders such as major depressive disorder (MDD) and bipolar disorder 
      (BD). METHODS: Genomic DNA was derived from peripheral blood mononuclear cells 
      (PBMCs) and was bisulfite converted. Percentage of methylated reference (PMR) was 
      calculated based on results from quantitative real-time PCR following the 
      MethyLight protocol. For statistical analysis parametric procedures were 
      performed as appropriate. RESULTS: In this study 544 subjects were included, 207 
      MDD subjects, 59 BD subjects and 278 control subjects. The BDNF exon I promoter 
      methylation resulted to be significantly increased in MDD subjects compared to BD 
      subjects (p=0.0089) and control subjects (p<0.001). Furthermore, the increase of 
      methylation in MDD subjects was significantly associated with AD therapy 
      (p=0.0019) but not to the clinical features of depression such as the severity of 
      symptoms (p=n.s.). None of the 12 investigated single nucleotide polymorphisms 
      (SNP) showed significant genotype-methylation interactions. LIMITATIONS: Although 
      based on previous findings, the DNA methylation was evaluated within only one CpG 
      island of the different alternative BDNF gene transcripts. CONCLUSIONS: The 
      results suggest that the methylation status might not only be affected by the 
      disease phenotype but might also be further influenced by pharmacological 
      treatment, therefore harbouring the possibility of identifying new insights for 
      treatment options.
CI  - Copyright (c) 2014. Published by Elsevier B.V.
FAU - Carlberg, Laura
AU  - Carlberg L
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Scheibelreiter, Janine
AU  - Scheibelreiter J
AD  - Clinical Institute of Pathology, Medical University of Vienna, Austria.
FAU - Hassler, Melanie R
AU  - Hassler MR
AD  - Clinical Institute of Pathology, Medical University of Vienna, Austria.
FAU - Schloegelhofer, Monika
AU  - Schloegelhofer M
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Schmoeger, Michaela
AU  - Schmoeger M
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Ludwig, Birgit
AU  - Ludwig B
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Kasper, Siegfried
AU  - Kasper S
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Aschauer, Harald
AU  - Aschauer H
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria.
FAU - Egger, Gerda
AU  - Egger G
AD  - Clinical Institute of Pathology, Medical University of Vienna, Austria.
FAU - Schosser, Alexandra
AU  - Schosser A
AD  - Department of Psychiatry and Psychotherapy, Medical University of Vienna, 
      Wahringer Gurtel 18-20, 1090 Vienna, Austria; Zentrum fur Seelische Gesundheit 
      LEOpoldau, Vienna, Austria. Electronic address: 
      alexandra.schosser@meduniwien.ac.at.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140719
PL  - Netherlands
TA  - J Affect Disord
JT  - Journal of affective disorders
JID - 7906073
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adult
MH  - Bipolar Disorder/*genetics
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - CpG Islands
MH  - *DNA Methylation
MH  - Depressive Disorder, Major/*genetics
MH  - *Epigenesis, Genetic
MH  - Exons
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
OTO - NOTNLM
OT  - Affective disorders
OT  - Antidepressant therapy
OT  - BDNF methylation
OT  - Depression
OT  - Epigenetics
OT  - Genetic association study
EDAT- 2014/08/12 06:00
MHDA- 2015/05/29 06:00
CRDT- 2014/08/09 06:00
PHST- 2014/01/29 00:00 [received]
PHST- 2014/06/16 00:00 [revised]
PHST- 2014/07/12 00:00 [accepted]
PHST- 2014/08/09 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
AID - S0165-0327(14)00453-4 [pii]
AID - 10.1016/j.jad.2014.07.022 [doi]
PST - ppublish
SO  - J Affect Disord. 2014 Oct;168:399-406. doi: 10.1016/j.jad.2014.07.022. Epub 2014 
      Jul 19.