PMID- 25108114
OWN - NLM
STAT- MEDLINE
DCOM- 20150110
LR  - 20211021
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 464-465
DP  - 2014 Sep
TI  - Antigenic structures stably expressed by recombinant TGEV-derived vectors.
PG  - 274-286
LID - S0042-6822(14)00335-3 [pii]
LID - 10.1016/j.virol.2014.07.027 [doi]
AB  - Coronaviruses (CoVs) are positive-stranded RNA viruses with potential as 
      immunization vectors, expressing high levels of heterologous genes and eliciting 
      both secretory and systemic immune responses. Nevertheless, its high 
      recombination rate may result in the loss of the full-length foreign gene, 
      limiting their use as vectors. Transmissible gastroenteritis virus (TGEV) was 
      engineered to express porcine reproductive and respiratory syndrome virus (PRRSV) 
      small protein domains, as a strategy to improve heterologous gene stability. 
      After serial passage in tissue cultures, stable expression of small PRRSV protein 
      antigenic domains was achieved. Therefore, size reduction of the heterologous 
      genes inserted in CoV-derived vectors led to the stable expression of antigenic 
      domains. Immunization of piglets with these TGEV vectors led to partial 
      protection against a challenge with a virulent PRRSV strain, as immunized animals 
      showed reduced clinical signs and lung damage. Further improvement of 
      TGEV-derived vectors will require the engineering of vectors with decreased 
      recombination rate.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Becares, Martina
AU  - Becares M
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Molecular and Cell 
      Biology, Campus Universidad Autonoma de Madrid, Darwin 3, Madrid 28049, Spain.
FAU - Sanchez, Carlos M
AU  - Sanchez CM
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Molecular and Cell 
      Biology, Campus Universidad Autonoma de Madrid, Darwin 3, Madrid 28049, Spain.
FAU - Sola, Isabel
AU  - Sola I
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Molecular and Cell 
      Biology, Campus Universidad Autonoma de Madrid, Darwin 3, Madrid 28049, Spain.
FAU - Enjuanes, Luis
AU  - Enjuanes L
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Molecular and Cell 
      Biology, Campus Universidad Autonoma de Madrid, Darwin 3, Madrid 28049, Spain. 
      Electronic address: L.Enjuanes@cnb.csic.es.
FAU - Zuniga, Sonia
AU  - Zuniga S
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Molecular and Cell 
      Biology, Campus Universidad Autonoma de Madrid, Darwin 3, Madrid 28049, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140809
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/administration & dosage/chemistry/*genetics/*immunology
MH  - *Gene Expression
MH  - Genetic Vectors/*genetics/metabolism
MH  - Immunization
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/genetics/*immunology
MH  - Swine
MH  - Transmissible gastroenteritis virus/*genetics/metabolism
MH  - Viral Vaccines/administration & dosage/chemistry/genetics/immunology
PMC - PMC7112069
OTO - NOTNLM
OT  - Coronavirus derived vectors
OT  - PRRSV
OT  - Positive-strand RNA viruses
OT  - RNA vector stability
OT  - TGEV
EDAT- 2014/08/12 06:00
MHDA- 2015/01/13 06:00
PMCR- 2014/08/09
CRDT- 2014/08/10 06:00
PHST- 2014/05/27 00:00 [received]
PHST- 2014/06/17 00:00 [revised]
PHST- 2014/07/17 00:00 [accepted]
PHST- 2014/08/10 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
PHST- 2014/08/09 00:00 [pmc-release]
AID - S0042-6822(14)00335-3 [pii]
AID - 10.1016/j.virol.2014.07.027 [doi]
PST - ppublish
SO  - Virology. 2014 Sep;464-465:274-286. doi: 10.1016/j.virol.2014.07.027. Epub 2014 
      Aug 9.