PMID- 25108738
OWN - NLM
STAT- MEDLINE
DCOM- 20150707
LR  - 20141108
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 70
IP  - 3
DP  - 2014 Dec
TI  - Effects of mild and moderate hypothemia therapy on expression of cerebral neuron 
      apoptosis related proteins and glial fiber acidic protein after rat 
      cardio-pulmonary resuscitation.
PG  - 1519-25
LID - 10.1007/s12013-014-0087-1 [doi]
AB  - To explore the effects of different degrees of hypothermia on brain tissue 
      apoptosis after cardio-pulmonary resuscitation (CPR). Cardiac arrest for 5 min 
      induced by asphyxia method was used to create CPR model. 30 SD rats were randomly 
      divided into control group (normothermia), 33  degrees C hypothermia group and 30  degrees C 
      hypothermia group with ten rats in each. Rats in control group received routine 
      treatment at 25  degrees C room temperature after CPR; Rats in mild hypothermia and 
      moderate hypothermia groups were given hypothermia treatment 0.5 h after CPR. 
      Brain tissue in all groups was taken 24 h after CPR, and immunohistochemistry was 
      used to detect the caspase-3 in cerebral cortex and glial fiber acidic protein 
      (GFAP) expression in astrocyte. Western blotting was used to detect Bcl-2 and Bax 
      protein expression, and histopathological change was observed in brain tissue. 
      Compare to the control group, caspase-3 expression in cerebral neurons in 
      hypothermia group was significantly decreased (p<0.01), which was significantly 
      lower in 30  degrees C group than that in 33  degrees C group (p > 0.05); GFAP level in 
      hypothermia groups was significantly increased (p < 0.01), which was higher in 30 
       degrees C hypothermia group than that in 33  degrees C hypothermia group (p < 0.05); Bcl-2 
      expression level in hypothermia group was significantly increased (p < 0.01), 
      which was higher in 30  degrees C hypothermia group than that in 33  degrees C hypothermia group 
      (p < 0.05); The level of Bax had no significant difference among the three 
      groups. Hypothermia-regulated GFAP expression by decreasing caspase-3 expression 
      and increasing Bcl-2 expression to promote brain cell signaling transduction, and 
      further inhibited cell apoptosis and reduced brain injury. Moderate hypothermia 
      therapy is more effective than mild hypothermia in preventing brain injure.
FAU - Ding, Li
AU  - Ding L
AD  - Department of Anesthesiology, The First Affiliated Hospital of Soochow 
      University, 188 Shizi Street, Suzhou, 215006, Jiangsu, China.
FAU - Gao, Xiang
AU  - Gao X
FAU - Yu, Shenghui
AU  - Yu S
FAU - Yang, Jianping
AU  - Yang J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Apoproteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Apoproteins/*metabolism
MH  - Brain/*metabolism
MH  - Brain Injuries/*metabolism/*therapy
MH  - Cardiopulmonary Resuscitation/*methods
MH  - Cells, Cultured
MH  - Combined Modality Therapy/methods
MH  - Gene Expression Regulation
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - Hyperthermia, Induced/*methods
MH  - Male
MH  - Neurons/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Treatment Outcome
EDAT- 2014/08/12 06:00
MHDA- 2015/07/08 06:00
CRDT- 2014/08/11 06:00
PHST- 2014/08/11 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/07/08 06:00 [medline]
AID - 10.1007/s12013-014-0087-1 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2014 Dec;70(3):1519-25. doi: 10.1007/s12013-014-0087-1.