PMID- 25109657
OWN - NLM
STAT- MEDLINE
DCOM- 20150526
LR  - 20161125
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 34
IP  - 4
DP  - 2014 Oct
TI  - Anti-inflammatory potential of peat moss extracts in 
      lipopolysaccharide-stimulated RAW 264.7 macrophages.
PG  - 1101-9
LID - 10.3892/ijmm.2014.1881 [doi]
AB  - The aim of the present study was to identify the anti-inflammatory and 
      anti-oxidative effects of peat moss aqueous extract (PME) on lipopolysaccharide 
      (LPS)-stimulated RAW 264.7 macrophages. To demonstrate the anti-inflammatory and 
      antioxidant effects of PME, the levels of nitric oxide (NO) and cytokines were 
      measured using Griess reagent and cytokine ELISA kits, respectively. Reverse 
      transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis were 
      conducted to evaluate the expression of genes and proteins. Immunofluorescence 
      was used to measure the expression and translocation of transcription factors. 
      Pre-treatment with PME inhibited the production of prostaglandin E(2) and NO by 
      suppressing the gene expression of cyclooxygenase-2 and inducible NO synthase, 
      respectively. The LPS-stimulated gene expression and the production of tumor 
      necrosis factor-alpha and interleukin-1beta were significantly reduced by PME. In the 
      LPS-stimulated RAW 264.7 cells, nuclear factor‑kappaB (NF-kappaB) translocated from the 
      cytosol to the nucleus, while pre-treatment with PME induced the sequestration of 
      NF-kappaB in the cytosol through the inhibition of IkappaBalpha degradation. In the same 
      manner, PME contributed to the inhibition of the activation of mitogen-activated 
      protein kinases. In addition, the PME-treated RAW 264.7 cells facilitated the 
      activation of nuclear factor-like 2 (Nrf2) , and in turn, enhanced heme 
      oxygenase-1 (HO-1) expression. These results indicate that PME exerts 
      anti-inflammatory and antioxidant effects, and suggest that PME may neutralize 
      inflammation and prevent cellular damage by oxidative stress.
FAU - Choi, Woo-Suk
AU  - Choi WS
AD  - Department of Microbiology, College of Natural Sciences, Pukyong National 
      University, Busan 608-737, Republic of Korea.
FAU - Jeong, Jin-Woo
AU  - Jeong JW
AD  - Department of Biochemistry, Dongeui University College of Oriental Medicine, 
      Busan 614-052, Republic of Korea.
FAU - Kim, Sung Ok
AU  - Kim SO
AD  - Department of Herbal Pharmacology, Daegu Haany University College of Oriental 
      Medicine, Daegu 706-828, Republic of Korea.
FAU - Kim, Gi-Young
AU  - Kim GY
AD  - Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National 
      University, Jeju 690-756, Republic of Korea.
FAU - Kim, Byung-Woo
AU  - Kim BW
AD  - Anti-Aging Research Center and Blue-Bio Industry RIC, Dongeui University, Busan 
      614-714, Republic of Korea.
FAU - Kim, Cheol Min
AU  - Kim CM
AD  - Department of Biochemistry, Busan National University College of Medicine, 
      Yangsan 626-870, Republic of Korea.
FAU - Seo, Yong-Bae
AU  - Seo YB
AD  - Department of Microbiology, College of Natural Sciences, Pukyong National 
      University, Busan 608-737, Republic of Korea.
FAU - Kim, Woe-Yeon
AU  - Kim WY
AD  - Division of Applied Life Science, Plant Molecular Biology and Biotechnology 
      Research Center, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Lee, Sang-Yeol
AU  - Lee SY
AD  - Division of Applied Life Science, Plant Molecular Biology and Biotechnology 
      Research Center, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Jo, Kwon-Ho
AU  - Jo KH
AD  - Green Vortex Co., Ltd., Busanjin-gu, Busan 614-714, Republic of Korea.
FAU - Choi, Young Ju
AU  - Choi YJ
AD  - Department of Food and Nutrition, College of Medical Life Sciences, Silla 
      University, Busan 617-736, Republic of Korea.
FAU - Choi, Yung Hyun
AU  - Choi YH
AD  - Department of Biochemistry, Dongeui University College of Oriental Medicine, 
      Busan 614-052, Republic of Korea.
FAU - Kim, Gun-Do
AU  - Kim GD
AD  - Department of Microbiology, College of Natural Sciences, Pukyong National 
      University, Busan 608-737, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140806
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Death/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cytokines/metabolism
MH  - Dinoprostone/metabolism
MH  - Enzyme Activation/drug effects
MH  - Heme Oxygenase-1/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/drug effects/enzymology/*metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-E2-Related Factor 2/metabolism
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Sphagnopsida/*chemistry
EDAT- 2014/08/12 06:00
MHDA- 2015/05/27 06:00
CRDT- 2014/08/12 06:00
PHST- 2014/03/07 00:00 [received]
PHST- 2014/07/17 00:00 [accepted]
PHST- 2014/08/12 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/05/27 06:00 [medline]
AID - 10.3892/ijmm.2014.1881 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Oct;34(4):1101-9. doi: 10.3892/ijmm.2014.1881. Epub 2014 Aug 
      6.