PMID- 25109926
OWN - NLM
STAT- MEDLINE
DCOM- 20150522
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 10
IP  - 4
DP  - 2014 Oct
TI  - Decreased osteoclastogenesis, osteoblastogenesis and low bone mass in a mouse 
      model of type 2 diabetes.
PG  - 1935-41
LID - 10.3892/mmr.2014.2430 [doi]
AB  - The effect of type 2 diabetes mellitus (T2DM) on bone is controversial. 
      Therefore, the present study investigated whether T2DM causes osteoporosis and 
      explored the underlying mechanisms involved in this process. The effects of T2DM 
      on bone physiology were analyzed in a mouse model of T2DM; KK/Upj‑Ay/J (KK‑Ay) 
      mice develop diabetes after 8 weeks and exhibit stable diabetes symptoms and 
      signs after 10 weeks when fed a KK‑Ay mouse maintenance fodder. Diabetic mice 
      exhibited hyperglycemia, hyperinsulinemia and increased body and fat pad weight 
      in comparison with C57BL/6 non-diabetic mice. Furthermore, diabetic mice 
      demonstrated low bone weight and bone mineral density in the femur, tibia and 
      fifth lumbar vertebra. Using von Kossa and tartrate-resistant acid phosphatase 
      (TRAP) staining, alkaline phosphatase and TRAP activity analyses and gene 
      profiling it was demonstrated that osteoblastogenesis and osteoclastogenesis were 
      impaired in diabetic mice. To evaluate the bone biomechanics, the ultimate load 
      of the bone was analyzed. It was found that the ultimate load of the tibia in 
      diabetic mice was lower than that in the controls. The results from the present 
      study suggest that bone metabolism is impaired in T2DM, resulting in decreased 
      osteoblastogenesis, osteoclastogenesis and bone mass.
FAU - Xu, Fei
AU  - Xu F
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Dong, Yonghui
AU  - Dong Y
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Huang, Xin
AU  - Huang X
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Li, Mi
AU  - Li M
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Qin, Liang
AU  - Qin L
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Ren, Ye
AU  - Ren Y
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Guo, Fengjing
AU  - Guo F
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Chen, Anmin
AU  - Chen A
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
FAU - Huang, Shilong
AU  - Huang S
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430000, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140730
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Isoenzymes)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Acp5 protein, mouse)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Density
MH  - Bone and Bones/chemistry/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Experimental/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Femur/cytology
MH  - Isoenzymes/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Osteogenesis
MH  - Spine/diagnostic imaging
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Tibia/diagnostic imaging
MH  - Tomography, X-Ray Computed
EDAT- 2014/08/12 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/08/12 06:00
PHST- 2013/09/21 00:00 [received]
PHST- 2013/10/25 00:00 [accepted]
PHST- 2014/08/12 06:00 [entrez]
PHST- 2014/08/12 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.3892/mmr.2014.2430 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Oct;10(4):1935-41. doi: 10.3892/mmr.2014.2430. Epub 2014 Jul 
      30.